Jie Zhang

Bio: Jie Zhang is an academic researcher from East China University of Science and Technology. The author has contributed to research in topics: Medicine & Large Hadron Collider. The author has an hindex of 178, co-authored 4857 publications receiving 221720 citations. Previous affiliations of Jie Zhang include University of Bedfordshire & CERN.

Pharmacologic inhibition of poly(ADP-ribose) polymerase is neuroprotective following traumatic brain injury in rats

Abstract: The nuclear enzyme poly(ADP-ribose) polymerase (PARP), which has been shown to be activated following experimental traumatic brain injury (TBI), binds to DNA strand breaks and utilizes nicotinamide adenine dinucleotide (NAD) as a substrate. Since consumption of NAD may be deleterious to recovery in the setting of CNS injury, we examined the effect of a potent PARP inhibitor, GPI 6150, on histological outcome following TBI in the rat. Rats (n = 16) were anesthetized, received a preinjury dose of GPI 6150 (30 min; 15 mg/kg, i.p.), subjected to lateral fluid percussion (FP) brain injury of moderate severity (2.5-2.8 atm), and then received a second dose 3 h postinjury (15 mg/kg, i.p.). Lesion area was examined using Nissl staining, while DNA fragmentation and apoptosis-associated cell death was assessed with terminal deoxynucleotidyl-transferase-mediated biotin-dUTP nick end labeling (TUNEL) with stringent morphological evaluation. Twenty-four hours after brain injury, a significant cortical lesion and number of TUNEL-positive/nonapoptotic cells and TUNEL-positive/apoptotic cells in the injured cortex of vehicle-treated animals were observed as compared to uninjured rats. The size of the trauma-induced lesion area was significantly attenuated in the GPI 6150-treated animals versus vehicle-treated animals (p < 0.05). Treatment of GPI 6150 did not significantly affect the number of TUNEL-positive apoptotic cells in the injured cortex. The observed neuroprotective effects on lesion size, however, offer a promising option for further evaluation of PARP inhibition as a means to reduce cellular damage associated with TBI.

Search for vectorlike charge 2/3 T quarks in proton-proton collisions at √(s) = 8 TeV

Abstract: A search for fermionic top quark partners T of charge 2/3 is presented. The search is carried out in proton-proton collisions corresponding to an integrated luminosity of 19.7 inverse femtobarns collected at a center-of-mass energy of sqrt(s) = 8 TeV with the CMS detector at the LHC. The T quarks are assumed to be produced strongly in pairs and can decay into tH, tZ, and bW. The search is performed in five exclusive channels: a single-lepton channel, a multilepton channel, two all-hadronic channels optimized either for the bW or the tH decay, and one channel in which the Higgs boson decays into two photons. The results are found to be compatible with the standard model expectations in all the investigated final states. A statistical combination of these results is performed and lower limits on the T quark mass are set. Depending on the branching fractions, lower mass limits between 720 and 920 GeV at 95% confidence level are found. These are among the strongest limits on vector-like T quarks obtained to date.

Measurement of the B→Xsγ branching fraction and photon energy spectrum using the recoil method

Abstract: We present a measurement of the branching fraction and photon-energy spectrum for the decay B -> X-s gamma using data from the BABAR experiment. The data sample corresponds to an integrated luminosity of 210 fb(-1), from which approximately 680 000 B (B) over bar events are tagged by a fully reconstructed hadronic decay of one of the B mesons. In the decay of the second B meson, an isolated high-energy photon is identified. We measure B(B -> X-s gamma) = (3.66 +/- 0.85(stat) +/- 0.60(syst)) x 10(-4) for photon energies E-gamma above 1.9 GeV in the B rest frame. From the measured spectrum we calculate the first and second moments for different minimum photon energies, which are used to extract the heavy-quark parameters m(b) and mu(2)(pi). In addition, measurements of the direct CP asymmetry and isospin asymmetry are presented.

Search for resonant and nonresonant Higgs boson pair production in the b(b)over-barl nu l nu final state in proton-proton collisions at root s=13 TeV

Abstract: Searches for resonant and nonresonant pair-produced Higgs bosons (HH) decaying respectively into l nu l nu, through either W or Z bosons, and b (b) over bar are presented The analyses are based on a sample of proton-proton collisions at root s = 13 TeV, collected by the CMS experiment at the LHC, corresponding to an integrated luminosity of 359 fb(-1) Data and predictions from the standard model are in agreement within uncertainties For the standard model HH hypothesis, the data exclude at 95% confidence level a product of the production cross section and branching fraction larger than 72 fb, corresponding to 79 times the standard model prediction Constraints are placed on different scenarios considering anomalous couplings, which could affect the rate and kinematics of HH production Upper limits at 95% confidence level are set on the production cross section of narrow-width spin-0 and spin-2 particles decaying to Higgs boson pairs, the latter produced with minimal gravity-like coupling

I and i

Abstract: There is, I think, something ethereal about i —the square root of minus one. I remember first hearing about it at school. It seemed an odd beast at that time—an intruder hovering on the edge of reality. Usually familiarity dulls this sense of the bizarre, but in the case of i it was the reverse: over the years the sense of its surreal nature intensified. It seemed that it was impossible to write mathematics that described the real world in …

A new mathematical model for relative quantification in real-time RT-PCR.

Abstract: Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to becoming a routine tool in molecular biology to study low abundance gene expression. Real-time PCR is easy to perform, provides the necessary accuracy and produces reliable as well as rapid quantification results. But accurate quantification of nucleic acids requires a reproducible methodology and an adequate mathematical model for data analysis. This study enters into the particular topics of the relative quantification in real-time RT–PCR of a target gene transcript in comparison to a reference gene transcript. Therefore, a new mathematical model is presented. The relative expression ratio is calculated only from the real-time PCR efficiencies and the crossing point deviation of an unknown sample versus a control. This model needs no calibration curve. Control levels were included in the model to standardise each reaction run with respect to RNA integrity, sample loading and inter-PCR variations. High accuracy and reproducibility (<2.5% variation) were reached in LightCycler PCR using the established mathematical model.