Jinge Li

Bio: Jinge Li is an academic researcher from Henan University. The author has contributed to research in topics: Peptide nucleic acid & Polymerization. The author has an hindex of 4, co-authored 9 publications receiving 41 citations.

Polymer-functionalized carbon nanotubes prepared via ring-opening polymerization for electrochemical detection of carcinoembryonic antigen

Abstract: Carcinoembryonic antigen (CEA) is regarded as the most common tumor biomarker, the precise and sensitive measurement of which has great significance for the cancer diagnosis, treatment, and prognosis. Herein, for the first time, we construct a novel sandwich-type electrochemical aptasensor for CEA detection based on polymer-functionalized carbon nanotubes (CNTs) composites prepared by ring-opening polymerization (ROP). CEA-aptamer 1 (Apt 1) is self-assembled on a gold electrode surface and used as a probe for CEA capture. CNTs are used as the substrates for polymer chain growth to prepare poly(ferrocenyl glycidyl ether)-grafted CNTs (PFcGE-CNTs) composites by ROP. Due to the efficient polymerization and good biocompatibility of ROP technology, PFcGE-CNTs composites can provide a hydrophilic-biocompatible nanoplatform for CEA-aptamer 2 (Apt 2) capture and serve as a signal amplifier. Remarkably, PFcGE-CNTs composites feature the characteristics of good conductivity and abundant ferrocene derivatives, which significantly amplify the electrochemical signal. Under optimized conditions, the fabricated aptasensor shows a wide detection range of 1 × 10−15–1 × 10−8 g mL−1 and a limit of detection (LOD) down to 2.8 × 10−16 g mL−1, which are superior to those of most reported electrochemical methods. Furthermore, the proposed aptasensor exhibits excellent applicability for CEA detection in practical sample analysis, which is of promising application potential for the early diagnosis and monitoring of cancer.

Electrochemical ultrasensitive detection of CYFRA21-1 using Ti 3 C 2 T x -MXene as enhancer and covalent organic frameworks as labels

Abstract: The concentration level of cytokeratin fragment antigen 21-1 (CYFRA21-1) can be used as an important indicator for predicting non–small cell lung cancer (NSCLC). Here, a sandwich-type electrochemical immunosensor for ultrasensitive detection of CYFRA21-1 is developed. The sensor based on a combination of gold nanoparticle (AuNPs) decorated Ti3C2Tx-MXene (Au-Ti3C2Tx) as the substrate enhancer, and toluidine blue (TB) modified AuNPs doped covalent organic framework (COF) polymer as the signal tag (TB-Au-COF). The Au-Ti3C2Tx is used to capture numerous primary antibodies and accelerate the electron transfer rate of the substrate, while the TB-Au-COF can be applied to provide a large number of signal units TB and secondary antibodies. These features of composites endow the proposed immunosensor with high sensitivity and current response to CYFRA21-1. Under optimum conditions, the immunosensor offers a wide current response for CYFRA21-1 from 0.5–1.0 × 104 pg·mL−1 with a detection limit of 0.1 pg·mL−1. Furthermore, the biosensing platform can be applied for CYFRA21-1 detection to analyze real serum samples, providing an effective and useful avenue for the applicability of Au-Ti3C2Tx and TB-Au-COF composite materials in biosensing field.

Polymer-functionalized carbon nanotubes prepared via ring-opening polymerization for electrochemical detection of carcinoembryonic antigen

Abstract: Carcinoembryonic antigen (CEA) is regarded as the most common tumor biomarker, the precise and sensitive measurement of which has great significance for the cancer diagnosis, treatment, and prognosis. Herein, for the first time, we construct a novel sandwich-type electrochemical aptasensor for CEA detection based on polymer-functionalized carbon nanotubes (CNTs) composites prepared by ring-opening polymerization (ROP). CEA-aptamer 1 (Apt 1) is self-assembled on a gold electrode surface and used as a probe for CEA capture. CNTs are used as the substrates for polymer chain growth to prepare poly(ferrocenyl glycidyl ether)-grafted CNTs (PFcGE-CNTs) composites by ROP. Due to the efficient polymerization and good biocompatibility of ROP technology, PFcGE-CNTs composites can provide a hydrophilic-biocompatible nanoplatform for CEA-aptamer 2 (Apt 2) capture and serve as a signal amplifier. Remarkably, PFcGE-CNTs composites feature the characteristics of good conductivity and abundant ferrocene derivatives, which significantly amplify the electrochemical signal. Under optimized conditions, the fabricated aptasensor shows a wide detection range of 1 × 10−15–1 × 10−8 g mL−1 and a limit of detection (LOD) down to 2.8 × 10−16 g mL−1, which are superior to those of most reported electrochemical methods. Furthermore, the proposed aptasensor exhibits excellent applicability for CEA detection in practical sample analysis, which is of promising application potential for the early diagnosis and monitoring of cancer.

Recent achievements and challenges on nanomaterial based electrochemical biosensors for the detection of colon and lung cancer biomarkers

Abstract: Cancer is still one of the leading diseases and causes of death in the world. More than 200 types of cancers are currently known. Early diagnosis still is an important integral part of cancer treatment. The detection of cancer biomarkers plays an essential role in clinical diagnosis and early treatment for patients. Lung and colon cancers are the most common disease. Still, they are a major cause of cancer-related deaths globally due to their difficult diagnosis in early stages resulting in late treatment. Colon cancer tumors frequently metastasize to the lung. However, identifying biomarkers such as secretory proteins is an attractive way to monitor the lung and colon cancer progression in patients at earlier stages. Nowadays, many efforts have been invested in biomarker discovery that can provide a sensitive and low-cost sensor technology using nanomaterials for non-invasive disease detection. Numerous attractive biomarker candidates such as DNA, RNA, mRNA, aptamers, metabolomics biomolecules, enzymes, and proteins can be utilized for the early diagnosis of lung and colon cancer. As the detection devices are generally highly sensitive, simple preparation, and rapid response, electrochemical biosensors are increasingly used to detect cancer markers. Many electroanalytical methods are developed for the detection of lung and colon cancer biomarkers. So, in this paper, the recent advances and improvements (2011–2021) in nanomaterials based electrochemical biosensors for the detection of the lung and colon cancer biomarkers are reviewed.