Molecular characterization of a new fusarivirus infecting Botryosphaeria dothidea, the causal agent of pear ring rot disease

The complete genome sequence of a novel single-stranded [+ ssRNA] positive-sense (+) RNA mycovirus, designated as "Pleurotus citrinopileatus ourmiavirus 1" (PcOV1), isolated from Pleurotus citrinopileatus strain CCMJ2141, was determined. The complete genome of PcOV1 is composed of 2,535 nucleotides. It contains a single open reading frame (ORF), which encodes a protein of 657 amino acids (aa) containing conserved domains of an RNA-dependent RNA polymerase (RdRp). Phylogenetic analysis based on RdRp sequences revealed that PcOV1 is a new member of the genus Ourmiavirus in the family Botourmiaviridae. This is the first virus from P. citrinopileatus to be characterized. 

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Complete genome sequence of a novel mycovirus from Pleurotus citrinopileatus

Here, we describe a novel ourmia-like virus, Botryosphaeria dothidea ourmia-like virus 2 (BdOLV2), derived from the phytopathogenic fungus Botryosphaeria dothidea strain ZM180192-1 infecting maize in Henan province of China. The complete genome sequence of BdOLV2 consists of a positive-sense single-stranded RNA (+ ssRNA) segment with a length of 2,532 nucleotides (nt). The sequence contains a large open reading frame (ORF) encoding a putative RNA-dependent RNA polymerase (RdRp) consisting of 605 amino acids (aa) with a molecular mass of 68.59 kDa. This RdRp protein contains eight typical conserved motifs associated with ourmia-like viruses. BLASTp analysis revealed that the RdRp protein of BdOLV2 had the highest similarity (62.10%, 58.15%, and 55.75% identity, respectively) to a virus previously identified as "Botourmiaviridae sp.", Macrophomina phaseolina ourmia-like virus 2, and Macrophomina phaseolina ourmia-like virus 2-A. Phylogenetic analysis based on the RdRp aa sequence indicated that BdOLV2 is a new member of the genus Magoulivirus in the family Botourmiaviridae. 

/pdf/molecular-characterization-of-a-novel-ourmia-like-virus-from-lmetp8tg.pdf

Molecular characterization of a novel ourmia‑like virus from the phytopathogenic fungus Botryosphaeria dothidea

Characterization of a novel magoulivirus isolated from the phytopathogenic fungus Verticillium dahlia

Abstract In this study, we report the complete genome sequence of a novel toti-like virus, tentatively named Rhizopus stolonifer toti-like virus 1 (RsTV1), identified from a phytopathogenic fungal agent of apple fruit rot disease, Rhizopus stolonifer strain A2-1. RsTV1 had a double-stranded RNA genome. The complete RsTV1 genome was 5178 nucleotides (nt) in length and contained two open reading frames (ORFs) encoding a putative coat protein (CP) and RNA-dependent RNA polymerase (RdRp). Phylogenetic analyses based on the RdRp and CP amino acid sequences revealed that RsTV1 was closely related to viruses of the unclassified totiviruses. In stress-inducing Vogel’s minimal and sodium dodecyl sulfate-containing media, the hyphal growth of A2-1 was suppressed, but the accumulation of RsTV1 RNA was increased, indicating that stresses promote RsTV1 replication. To our knowledge, this is the first report of a mycovirus found in R. stolonifer . 

/pdf/complete-genome-sequence-of-a-novel-toti-like-virus-2n593ewr.pdf

Complete genome sequence of a novel toti-like virus infecting the phytopathogenic fungus Rhizopus stolonifer

Here, we describe the full-length genome sequence of a novel ourmia-like mycovirus, tentatively named "Botryosphaeria dothidea ourmia-like virus 1" (BdOLV1), isolated from the phytopathogenic fungus Botryosphaeria dothidea strain 8A, associated with apple ring rot in Shanxi province, China. The complete BdOLV1 genome is comprised of a 2797-nucleotide positive-sense (+) single-stranded RNA (ssRNA) with a single open reading frame (ORF). The ORF putatively encodes a 642-amino-acid polypeptide with conserved RNA-dependent RNA polymerase (RdRp) motifs related to those of viruses of the family Botourmiaviridae. Phylogenetic analysis based on RdRp amino acid sequences showed that BdOLV1 is grouped with unclassified oomycete-infecting viruses closely related to members of the genus Botoulivirus in the family Botourmiaviridae. This is the first report of a novel (+)ssRNA virus in B. dothidea related to members of the genus Botoulivirus in the family Botourmiaviridae.

/pdf/complete-genome-sequence-of-a-novel-ourmia-like-mycovirus-4oandsso2s.pdf

Jingxian Luo

Papers

Complete genome sequence of a novel ourmia-like mycovirus infecting the phytopathogenic fungus Botryosphaeria dothidea.