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Jinwook Lee

Bio: Jinwook Lee is an academic researcher from Chung-Ang University. The author has contributed to research in topics: Cold storage & Preharvest. The author has an hindex of 17, co-authored 68 publications receiving 930 citations. Previous affiliations of Jinwook Lee include UPRRP College of Natural Sciences & United States Department of Agriculture.


Papers
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Journal ArticleDOI
TL;DR: In this paper, untargeted metabolic profiling with partial least squares analysis has been used to visualize changes in metabolic profile during hypoxic controlled atmosphere (CA) storage, ethylene insensitivity, and disorder development.
Abstract: ‘Empire’ apple fruit are more susceptible to flesh browning at 3.3°C if treated with 1-methylcyclopropene (1-MCP), an inhibitor of ethylene perception. To better understand the metabolic changes associated with this browning, untargeted metabolic profiling with partial least squares analysis has been used to visualize changes in metabolic profile during hypoxic controlled atmosphere (CA) storage, ethylene insensitivity, and disorder development. Overall, most carbohydrates and organic acids were not appreciably affected, but the levels of amino acids and volatile metabolites were significantly affected, by 1-MCP treatment. Sorbitol and levels of some amino acids were elevated towards the end of storage in 1-MCP treated fruit. CA storage reduced the levels of many volatile components and 1-MCP reduced these levels further. Additionally multiple metabolites were associated with the development of flesh browning symptoms. Unlike other volatile compounds, methanol levels gradually increased with storage duration, regardless of 1-MCP treatment, while 1-MCP decreased ethanol production. Results reveal metabolic changes during storage that may be associated with development of flesh browning symptoms.

117 citations

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TL;DR: The heated ginseng protected cell viability against H2O2-induced oxidative damage, and enhanced the activities of superoxide dismutase and catalase by dose dependently in V79-4 cells.

111 citations

Journal ArticleDOI
TL;DR: The results suggest that NIRS could be applied for the rapid determination of the contents of caffeine, EGC, C, E GCG, EC, ECG, and total catechins in tea leaves for breeding programs that develop high-quality tea plants.

86 citations

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TL;DR: In this paper, untargeted metabolic profiling using GC-MS and LC-MS was performed to characterize metabolism in cortex tissue of ‘Braeburn’ apple fruit treated with 2.g L−1 DPA or left untreated and stored under high CO2, controlled atmosphere (CA) storage of 1.5kPa O2/3kPa CO2 at 0.5°C for up to 12 weeks.

65 citations

Journal ArticleDOI
TL;DR: It is demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long- term Mg -deficiency.
Abstract: Magnesium (Mg)-deficiency is frequently observed in Citrus plantations and is responsible for the loss of productivity and poor fruit quality. Knowledge on the effects of Mg-deficiency on upstream targets is scarce. Seedlings of ‘Xuegan’ [Citrus sinensis (L.) Osbeck] were irrigated with Mg-deficient (0 mM MgSO4) or Mg-sufficient (1 mM MgSO4) nutrient solution for 16 weeks. Thereafter, we first investigated the proteomic responses of C. sinensis roots and leaves to Mg-deficiency using two-dimensional electrophoresis (2-DE) in order to (a) enrich our understanding of the molecular mechanisms of plants to deal with Mg-deficiency and (b) understand the molecular mechanisms by which Mg-deficiency lead to a decrease in photosynthesis. Fifty-nine upregulated and 31 downregulated protein spots were isolated in Mg-deficient leaves, while only 19 upregulated and 12 downregulated protein spots in Mg-deficient roots. Many Mg-deficiency-responsive proteins were involved in carbohydrate and energy metabolism, followed by protein metabolism, stress responses, nucleic acid metabolism, cell wall and cytoskeleton metabolism, lipid metabolism and cell transport. The larger changes in leaf proteome versus root one in response to Mg-deficiency was further supported by our observation that total soluble protein concentration was decreased by Mg-deficiency in leaves, but unaffected in roots. Mg-deficiency had decreased levels of proteins [i.e. ribulose-1,5-bisphosphate carboxylase (Rubisco), rubisco activase, oxygen evolving enhancer protein 1, photosynthetic electron transfer-like protein, ferredoxin-NADP reductase (FNR), aldolase] involved in photosynthesis, thus decreasing leaf photosynthesis. To cope with Mg-deficiency, C. sinensis leaves and roots might respond adaptively to Mg-deficiency through: improving leaf respiration and lowering root respiration, but increasing (decreasing) the levels of proteins related to ATP synthase in roots (leaves); enhancing the levels of proteins involved in reactive oxygen species (ROS) scavenging and other stress-responsive proteins; accelerating proteolytic cleavage of proteins by proteases, protein transport and amino acid metabolism; and upregulating the levels of proteins involved in cell wall and cytoskeleton metabolism. Our results demonstrated that proteomics were more affected by long-term Mg-deficiency in leaves than in roots, and that the adaptive responses differed between roots and leaves when exposed to long-term Mg-deficiency. Mg-deficiency decreased the levels of many proteins involved in photosynthesis, thus decreasing leaf photosynthesis.

56 citations


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Journal ArticleDOI
TL;DR: Focus is given to chloroplast ultrastructure, light-dependent and -independent reactions of photosynthesis and the diffusion of CO2 into chloroplasts and to the photoprotection of the photosynthetic apparatus.
Abstract: Potassium (K) and magnesium (Mg) are mineral nutrients that are required in large quantities by plants. Both elements critically contribute to the process of photosynthesis and the subsequent long-distance transport of photoassimilates. If K or Mg is not present in sufficient quantities in photosynthetic tissues, complex interactions of anatomical, physiological and biochemical responses result in a reduction of photosynthetic carbon assimilation. As a consequence, excessive production of reactive oxygen species causes photo-oxidation of the photosynthetic apparatus and causes an up-regulation of photoprotective mechanisms. In this article, we review the functioning of K and Mg in processes directly or indirectly associated with photosynthesis. Focus is given to chloroplast ultrastructure, light-dependent and -independent reactions of photosynthesis and the diffusion of CO2 - a major substrate for photosynthesis - into chloroplasts. We further emphasize their contribution to phloem-loading and long-distance transport of photoassimilates and to the photoprotection of the photosynthetic apparatus.

274 citations

Journal ArticleDOI
TL;DR: The enzymatic pretreatment as one of the biological pret treatment methods which has received less attention in the literature than the other pretreatment methods is reviewed, and the current status of research to improve the biogas rate and yield from the AD of lignocellulosic biomass via enzyme pretreatment is reviewed.

188 citations

Journal ArticleDOI
TL;DR: The widely documented intraspecies genetic variability regarding boron utilization efficiency and toxicity tolerance, together with the knowledge of the physiology and genetics of bor on, should result in the development of efficient and tolerant varieties that may represent a long-term sustainable solution for the problem of inadequate or excess borons supply.
Abstract: Boron is an essential plant micronutrient taken up via the roots mostly in the form of boric acid. Its important role in plant metabolism involves the stabilization of molecules with cis-diol groups. The element is involved in the cell wall and membrane structure and functioning; therefore, it participates in numerous ion, metabolite, and hormone transport reactions. Boron has an extremely narrow range between deficiency and toxicity, and inadequate boron supply exhibits a detrimental effect on the yield of agricultural plants. The deficiency problem can be solved by fertilization, whereas soil boron toxicity can be ameliorated using various procedures; however, these approaches are costly and time-consuming, and they often show temporary effects. Plant species, as well as the genotypes within the species, dramatically differ in terms of boron requirements; thus, the available soil boron which is deficient for one crop may exhibit toxic effects on another. The widely documented intraspecies genetic variability regarding boron utilization efficiency and toxicity tolerance, together with the knowledge of the physiology and genetics of boron, should result in the development of efficient and tolerant varieties that may represent a long-term sustainable solution for the problem of inadequate or excess boron supply.

174 citations