J
Jo Vandesompele
Researcher at Ghent University
Publications - 406
Citations - 67052
Jo Vandesompele is an academic researcher from Ghent University. The author has contributed to research in topics: Neuroblastoma & microRNA. The author has an hindex of 88, co-authored 383 publications receiving 59368 citations. Previous affiliations of Jo Vandesompele include Washington University in St. Louis & Ghent University Hospital.
Papers
More filters
Journal ArticleDOI
mRNA Capture Sequencing and RT-qPCR for the Detection of Pathognomonic, Novel, and Secondary Fusion Transcripts in FFPE Tissue: A Sarcoma Showcase
Anneleen Decock,David Creytens,Steve Lefever,Joni Van der Meulen,Jasper Anckaert,Ariane De Ganck,Jill Deleu,Bram De Wilde,Carolina Fierro,Scott Kuersten,Manuel Luypaert,Isabelle Rottiers,Gary P. Schroth,Sandra Steyaert,Katrien Vanderheyden,E. Vanden Eynde,Kimberly Verniers,Joke Verreth,J. Van Dorpe,Jo Vandesompele +19 more
TL;DR: This study shows that mRNA capture sequencing enhances the detection rate of pathognomonic fusions and enables the identification of novel and secondary fusion transcripts in sarcomas.
تاداشرإ MIQE )يقيقحلا تقولا في( يمكلا لسلستلما ه رملبلا لعافت براجت شرنل تامولعلما نم نىدلأا دحلا )RT-qPCR( )لسلستلما ةرملبلا لعافتل يقيقحلا تقولا(
Real-time qPCR as a tool for evaluating RNAi-mediated gene silencing
T Van Maerken,Pieter Mestdagh,Sarah De Clercq,Filip Pattyn,Nurten Yigit,Anne De Paepe,Jean-Christophe Marine,Frank Speleman,Jo Vandesompele +8 more
TL;DR: This article describes how rt-qPCR can be implemented as a tool to monitor silencing efficiency and functional effects of RNA interference (RNAi)-mediated gene knockdown, using examples from research on neuroblastoma.
Journal ArticleDOI
Recurrent chromosomal imbalances provide selective advantage to human embryonic stem cells under enhanced replicative stress conditions.
Liselot Mus,Stéphane Van Haver,Mina Popovic,Wim Trypsteen,Steve Lefever,Nadja Zeltner,Yudelca Ogando,Eva Jacobs,Geertrui Denecker,Ellen Sanders,Christophe Van Neste,Suzanne Vanhauwaert,Bieke Decaesteker,Dieter Deforce,Filip Van Nieuwerburgh,Pieter Mestdagh,Jo Vandesompele,Björn Menten,Katleen De Preter,Lorenz Studer,Björn Heindryckx,Kaat Durinck,Stephen S. Roberts,Frank Speleman +23 more
TL;DR: The data suggest that recurrent chromosomal segmental gains provide a proliferative advantage to hESCs under increased replicative stress, a process that may also explain the highly recurrent nature of certain imbalances in cancer.
Journal ArticleDOI
Selection and validation of reference genes for accurate RT-qPCR gene expression normalization in cacao beans during fermentation
Jocelyn De Wever,Jocelyn De Wever,Tibo De Coninck,Helena Everaert,Emmanuel Ohene Afoakwa,Frauke Coppieters,Hayley Rottiers,Stephen Yaw Opoku,S. T. Lowor,Koen Dewettinck,Jo Vandesompele,Jo Vandesompele,Steve Lefever,Steve Lefever,Kathy Messens +14 more
TL;DR: In this paper, an appropriate from bean to RT-qPCR protocol was developed for gene expression studies with reverse transcription quantitative PCR (RT-QPCR) in cacao beans.