J
John E. MacNair
Researcher at University of North Carolina at Chapel Hill
Publications - 3
Citations - 995
John E. MacNair is an academic researcher from University of North Carolina at Chapel Hill. The author has contributed to research in topics: Theoretical plate & Packed bed. The author has an hindex of 3, co-authored 3 publications receiving 976 citations.
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Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns.
TL;DR: F fused-silica capillaries with inner diameters of 30 microns are slurry packed with 1.5 microns nonporous octadecylsilane-modified silica particles to improve the efficiency and reduce analysis time for columns packed with small particles.
Journal ArticleDOI
Ultrahigh-Pressure Reversed-Phase Capillary Liquid Chromatography: Isocratic and Gradient Elution Using Columns Packed with 1.0-μm Particles
TL;DR: An electrically driven constant-flow syringe pump capable of generating mobile-phase pressures as high as 9000 bar (130,000 psi) is described, used in conjunction with an exponential dilution method for the gradient separation of peptides from a tryptic digest on a 27-cm-long capillary packed with 1.0-micron particles.
Journal ArticleDOI
Rapid separation and characterization of protein and peptide mixtures using 1.5 μm diameter non-porous silica in packed capillary liquid chromatography/mass spectrometry
TL;DR: This technique was applied to verify the existence of an overexpressed protein in an E. coli cell lysate and to confirm the presence of four glycoforms of a peptide generated in the proteolytic digest of an antibody.