J
John J. Mekalanos
Researcher at Harvard University
Publications - 382
Citations - 53371
John J. Mekalanos is an academic researcher from Harvard University. The author has contributed to research in topics: Vibrio cholerae & Cholera toxin. The author has an hindex of 118, co-authored 372 publications receiving 49816 citations. Previous affiliations of John J. Mekalanos include University of Texas Medical Branch & Broad Institute.
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A novel suicide vector and its use in construction of insertion mutations: osmoregulation of outer membrane proteins and virulence determinants in Vibrio cholerae requires toxR.
TL;DR: Certain environmental signals (i.e., osmolarity and the presence of amino acids) are tightly coupled to the expression of toxR-regulated proteins and therefore may be signals that are directly sensed by the ToxR protein.
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DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae
John F. Heidelberg,Jonathan A. Eisen,William C. Nelson,Rebecca A. Clayton,Michelle L. Gwinn,Robert J. Dodson,Daniel H. Haft,Erin Hickey,Jeremy Peterson,Lowell Umayam,Steven R. Gill,Karen E. Nelson,Timothy D. Read,Hervé Tettelin,Delwood Richardson,Maria D. Ermolaeva,Jessica Vamathevan,Steven Bass,Haiying Qin,Ioana Dragoi,Patrick Sellers,Lisa McDonald,Teresa Utterback,Robert D. Fleishmann,William C. Nierman,Owen White,Steven L. Salzberg,Hamilton O. Smith,Rita R. Colwell,Rita R. Colwell,John J. Mekalanos,J. Craig Venter,Claire M. Fraser +32 more
TL;DR: The V. cholerae genomic sequence provides a starting point for understanding how a free-living, environmental organism emerged to become a significant human bacterial pathogen.
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Lysogenic conversion by a filamentous phage encoding cholera toxin.
TL;DR: The emergence of toxigenic V. cholerae involves horizontal gene transfer that may depend on in vivo gene expression, and is shown here to be encoded by a filamentous bacteriophage (designated CTXΦ), which is related to coliphage M13.
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Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system
Stefan Pukatzki,Amy T. Ma,Derek Sturtevant,Bryan Krastins,David A. Sarracino,William C. Nelson,John F. Heidelberg,John J. Mekalanos +7 more
TL;DR: It is shown that vas genes are required for cytotoxicity of V. cholerae cells toward Dictyostelium amoebae and mammalian J774 macrophages by a contact-dependent mechanism, and it is proposed that these genes encode a prototypic “type VI” secretion system.
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Use of phoA gene fusions to identify a pilus colonization factor coordinately regulated with cholera toxin
TL;DR: It is concluded that the toxR gene plays a central role in the transcriptional regulation of multiple virulence genes of V. cholerae.