John Lyle

TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.

TL;DR: In this article, compositions of polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogue in the active site region are provided.

TL;DR: In this paper, the authors employ elements of the substrates in assisting the localization of active chemical groups in desired regions of the substrate, which may include optical, chemical and/or mechanical processes for the deposition, removal, activation, and deactivation of chemical groups.

TL;DR: In this article, two slow step systems can be produced by selecting the appropriate polymerase enzyme, reaction conditions including cofactors, and polymerase reaction substrates including the primed template and nucleotides.

TL;DR: In this article, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. But these methods are not suitable for the characterization of nucleic acids.