John Mcbride

TL;DR: Progress in developing CBP-enabling microorganisms is being made through two strategies: engineering naturally occurring cellulolytic microorganisms to improve product-related properties, such as yield and titer, and engineering non-cellulolytic organisms that exhibit high product yields and titers to express a heterologous cellulase system enabling cellulose utilization.

TL;DR: The economic motivation for CBP is addressed, advances and remaining obstacles forCBP organism development are reviewed, and the underlying fundamentals of microbial cellulose utilization are likely to be useful in order to guide the choice and development of CBP systems.

TL;DR: This review focuses on progress made toward the development of baker's yeast (Saccharomyces cerevisiae) for CBP, and the current status of saccharolytic enzyme expression in S. Cerevisiae to complement its natural fermentative ability is highlighted.

TL;DR: Gene or protein specific features and compatibility with the host are important for efficient cellobiohydrolase secretion in yeast and production of both CBH1 and CBH2 could be improved to levels where the barrier to CBH sufficiency in the hydrolysis of cellulose was overcome.

TL;DR: The results suggest that although heterologous CBHs can be produced in S. cerevisiae the titers of functionally secreted CBH are relatively low, and future studies should aim to increase the expression levels of CBHS.