Showing papers by "John Q. Trojanowski published in 1981"
TL;DR: The relative sensitivity of horseradish peroxidase (HRP) conjugates of cholera toxin and wheat germ agglutinin as orthogradely transported markers was compared with that of free HRP following unilateral, intravitreal injections of each probe in the rat.
146 citations
TL;DR: WGA-HRP is a more sensitive retrograde marker than free HRP at low concentrations in the rat visual system and binds specifically to moieties with terminalN-acetylglucosamine, but not freeHRP, is localized to neuronal GERL of striate cortex subsequent to endocytosis.
Abstract: The ability of horseradish peroxidase (HRP) and the lectin wheat germ agglutinin (WGA) covalently conjugated with HRP to label retrogradely dorsal lateral geniculate nucleus (dLGN) neurons, subsequent to injections of either marker into rat striate cortex, was assessed by counting labelled neurons in the dLGN. Rats injected with either marker in concentrations ranging from 0.1 to 100μg/μl of HRP either free or coupled to WGA were perfused 24 h later and their brains incubated using the chromagen tetramethyl benzidine. At high concentrations (2–100μg/μ1), comparable numbers of labelled neurons were observed in the dLGN but at low concentrations (0.1–1.0μg/μ1), WGA-HRP labelled 2–5 times more dLGN neurons than did unconjugated HRP. The sugarN-acetylglucosamine, and free WGA added in excess to WGA-HRP, abolished the retrograde labelling of dLGN neurons.
49 citations
TL;DR: It is concluded that the supranuclear neural elements mediating downgaze are situated within the confines of these lesions seen in the region of the thalamo-mesencephalic junction.
28 citations
TL;DR: A 7-day-old male infant with the hypoplastic right heart syndrome and clinically unsuspected acute appendicitis who died 7 days after the successful creation of a Waterston shunt is presented.
11 citations