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Showing papers by "John Q. Trojanowski published in 1984"


Journal ArticleDOI
TL;DR: Findings indicate that monoclonal antibodies against intermediate filament proteins such as neurofilaments and glial filaments retain their specificity and sensitivity when employed in paraffin sections in conjunction with the peroxidase-antiperoxidases method.

105 citations


Journal ArticleDOI
TL;DR: Intravitreal injections of WGHRP, CTHRP or BHRP all resulted in interneuronal transfer in the visual system, but there was no evidence for this in the tongue-hypoglossal nucleus pathway, and Native HRP did not undergo such transfer.

65 citations


Journal ArticleDOI
TL;DR: Alterations occurring in nerve proteins of transected nerves were studied in rat sciatic nerves using polyclonal and monoclonal antibodies to identify and monitor neurofilament (NF) epitopes among nerve proteins following their electrophoresis and transfer to nitrocellulose paper.
Abstract: Alterations occurring in nerve proteins of transected nerves were studied in rat sciatic nerves using polyclonal and monoclonal antibodies to identify and monitor neurofilament (NF) epitopes among nerve proteins following their electrophoresis and transfer to nitrocellulose paper. Immunoblot methods identified NF epitopes in NF triplet proteins (Mr 200,000, 150,000, and 68,000) and in NF nontriplet proteins (all other immunobands below Mr 200,000 and above Mr 40,000). NF triplet and nontriplet proteins were Triton-insoluble in both untransected and transected nerves. Extensive loss of NF triplet and most nontriplet proteins occurred during the 24-48-h period following nerve transection and was attributed to proteolytic degradation. Loss of protease-labile NF proteins led to a markedly reduced level of NF immunoreactivity in 2-day transected nerve. NF proteins which survived the 2-day posttransectional period were considered to represent protease-stable NF fragments. These fragments persisted in transected nerve for periods of at least 35 days. Most protease-stable NF fragments which retained immunoreactivity had Mr of 57,000-65,000. Low concentrations of the same immunobands were present in untransected nerves.

61 citations


Journal ArticleDOI
TL;DR: It is concluded that degenerating NF proteins have a distinctive granular appearance and that NF immunoreactivity can be demonstrated in NF breakdown products with immunohistochemical techniques for prolonged periods of time in rat and human nerves undergoing axonal degeneration.
Abstract: The fate of neurofilament (NF) proteins was examined in sections of nerves from rats that survived for 3 to 90 days following nerve transection, using anti-NF monoclonal antibodies and immunohistochemical techniques. The same methods were also applied to twenty-three human nerve biopsy specimens. Granular debris with NF immunoreactivity was observed in the rat nerves for up to 21 days after transection. Similar granular debris with NF protein immunoreactivity was observed in nerve biopsy speciments from patients with axonal degeneration. This change was most evident in specimens that demonstrated abundant ovoids in teased fiber preparations and in a patient with a recent traumatic nerve transection. Similar granular debris was not observed in normal rat or human nerves. We conclude that degenerating NF proteins have a distinctive granular appearance and that NF immunoreactivity can be demonstrated in NF breakdown products with immunohistochemical techniques for prolonged periods of time in rat and human nerves undergoing axonal degeneration. Immunohistochemical studies with anti-NF monoclonal antibodies offer a novel approach to the evaluation of human nerve biopsy specimens.

27 citations


Journal ArticleDOI
TL;DR: Monoclonal antibodies specific for neurofilaments (NF) and glial filaments (GF) were used to study the development of the normal human pituitary gland in fetuses, premature infants and adults and the expression of both NF and GF proteins correlated with maturation of the pituitaries.

25 citations


Journal Article
TL;DR: The results indicate that human teratomas can express all of these neural antigens, reflecting the presence of differentiated neurons, astrocytes, and oligodendroglia, respectively.
Abstract: Monoclonal antibodies specific for neurofilament proteins, glial filament protein, or myelin basic protein were used with immunohistochemistry for evaluation of a series of 14 human benign and malignant teratomas for the presence of these neural specific antigens The results indicate that human teratomas can express all of these neural antigens, reflecting the presence of differentiated neurons, astrocytes, and oligodendroglia, respectively Although the tumors were selected because neural tissue had been noted on microscopic examination, 2 of the 15 cases lacked all of these neural antigens, and both were malignant teratomas In the remaining cases, 2 or 3 of these neural antigens were detected The presence of all 3 was correlated with the presence of mature, and the absence of immature, neural elements The immunohistochemical detection of these antigens allows the confident recognition of neural elements in human teratomas, and their presence may be of prognostic significance

24 citations