Endocytosis of liposomes and intracellular fate of encapsulated molecules: Encounter with a low pH compartment after internalization in coated vesicles

Visualizing normal and defective bone development in zebrafish embryos using the fluorescent chromophore calcein

A Near‐Infrared Luminescent Label Based on YbIII Ions and Its Application in a Fluoroimmunoassay

Excerpt During the past 2 decades extraordinary advances have occurred in our understanding of the biology of normal cells. Dramatic progress has been made in defining the "molecular pathology" of ...

Metabolic Effects of Intracellular Infections in Man

Pressure homogenization of mammalian tissues.

Preparation and Properties of 3,6-Dihydroxy-2,4-bis-[N-N´-di-(carboxymethyl)-aminomethyl] fluoran

Summary The phospholipide content and the intracellular distribution of lipide phosphorus of Ehrlich ascites carcinoma cells have been studied. The lipides, 58 per cent of which are phospholipides, comprise 16 per cent of the dry weight. The nuclei contain 13.7 per cent of the lipide phosphorus; mitochondria, 29 per cent; microsomes, 52.5 per cent; and supernatant, 5.4 per cent. The phospholipides include 12.5 per cent acetal phosphatides and 81.93 per cent nitrogen-containing phospholipides; 3 per cent of the lipide phosphorus is contributed by phosphatidic acid and 4–16 per cent by inositol phosphatides. The molar ratio of nitrogen to phosphorus in Ehrlich ascites carcinoma phospholipides is 1.08. Choline contributes 53 per cent of the total nitrogen; ethanolamine, 30 per cent; sphingosine, 14 per cent; serine, 7 per cent; and amino acids other than serine, 3 per cent. About 6 per cent of the ethanolamine phosphatide appears to be in peptide linkage with serine and other amino acids. The significance of these findings is discussed in the light of the micromorphologic characteristics of ascites carcinoma cells.

Jon. Soderberg

Papers

Preparation and Properties of 3,6-Dihydroxy-2,4-bis-[N-N´-di-(carboxymethyl)-aminomethyl] fluoran

The phospholipides of Ehrlich ascites carcinoma cells: composition and intracellular distribution.