J
Jonathan S. Gootenberg
Researcher at Massachusetts Institute of Technology
Publications - 97
Citations - 37395
Jonathan S. Gootenberg is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: CRISPR & RNA. The author has an hindex of 40, co-authored 84 publications receiving 26663 citations. Previous affiliations of Jonathan S. Gootenberg include Harvard University & McGovern Institute for Brain Research.
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Journal ArticleDOI
Cpf1 is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system.
Bernd Zetsche,Jonathan S. Gootenberg,Omar O. Abudayyeh,Ian Slaymaker,Kira S. Makarova,Patrick Essletzbichler,Sara E. Volz,Julia Joung,John van der Oost,Aviv Regev,Aviv Regev,Eugene V. Koonin,Feng Zhang +12 more
TL;DR: In this paper, the authors characterized Cpf1, a putative class 2 CRISPR effector, which is a single RNA-guided endonuclease lacking tracrRNA and utilizes a T-rich protospacer-adjacent motif.
Journal ArticleDOI
Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
F. Ann Ran,Patrick D. Hsu,Chie Yu Lin,Jonathan S. Gootenberg,Silvana Konermann,Alexandro E. Trevino,David A. Scott,Azusa Inoue,Shogo Matoba,Yi Zhang,Feng Zhang +10 more
TL;DR: In this paper, an approach that combines a Cas9 nickase mutant with paired guide RNAs to introduce targeted double-strand breaks is described. But the approach is limited to mouse zygotes.
Journal ArticleDOI
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Omar O. Abudayyeh,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang +11 more
TL;DR: Structural-guided engineering of a CRISPR-Cas9 complex to mediate efficient transcriptional activation at endogenous genomic loci is described and the potential of Cas9-based activators as a powerful genetic perturbation technology is demonstrated.
Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity
F. Ann Ran,Patrick D. Hsu,Chie Yu Lin,Jonathan S. Gootenberg,Silvana Konermann,Alexandro E. Trevino,David A. Scott,Azusa Inoue,Shogo Matoba,Yi Zhang,Feng Zhang +10 more
TL;DR: It is demonstrated that using paired nicking can reduce off-target activity by 50- to 1,500-fold in cell lines and to facilitate gene knockout in mouse zygotes without sacrificing on-target cleavage efficiency.
Journal ArticleDOI
Nucleic acid detection with CRISPR-Cas13a/C2c2
Jonathan S. Gootenberg,Omar O. Abudayyeh,Jeong Wook Lee,Patrick Essletzbichler,Aaron J. Dy,Aaron J. Dy,Julia Joung,Vanessa Verdine,Nina M. Donghia,Nichole M. Daringer,Catherine A. Freije,Catherine A. Freije,Cameron Myhrvold,Cameron Myhrvold,Roby P. Bhattacharyya,Jonathan Livny,Aviv Regev,Aviv Regev,Eugene V. Koonin,Deborah T. Hung,Pardis C. Sabeti,James J. Collins,Feng Zhang +22 more
TL;DR: A Cas13a-based molecular detection platform, termed Specific High-Sensitivity Enzymatic Reporter UnLOCKing (SHERLOCK), is used to detect specific strains of Zika and Dengue virus, distinguish pathogenic bacteria, genotype human DNA, and identify mutations in cell-free tumor DNA.