J
José L. Avalos
Researcher at Princeton University
Publications - 65
Citations - 4555
José L. Avalos is an academic researcher from Princeton University. The author has contributed to research in topics: Metabolic engineering & NAD+ kinase. The author has an hindex of 20, co-authored 56 publications receiving 3576 citations. Previous affiliations of José L. Avalos include Johns Hopkins University School of Medicine & University of Washington.
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Journal ArticleDOI
A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family.
Jeffrey S. Smith,Carrie Baker Brachmann,Carrie Baker Brachmann,Ivana Celic,Margaret A. Kenna,Margaret A. Kenna,Shabazz Muhammad,Vincent J. Starai,José L. Avalos,Jorge C. Escalante-Semerena,Charles Grubmeyer,Cynthia Wolberger,Jef D. Boeke +12 more
TL;DR: The yeast Sir2 protein, required for transcriptional silencing, has an NAD+dependent histone deacetylase (HDA) activity that is eliminated in a yeast strain from which SIR2 and its four homologs have been deleted as mentioned in this paper.
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Mechanism of sirtuin inhibition by nicotinamide: altering the nad+ cosubstrate specificity of a sir2 enzyme
TL;DR: The characteristics of the altered specificity enzyme establish that Sir2 enzymes contain a single site that participates in catalysis and nicotinamide regulation and provides additional insights into the Sir2 catalytic mechanism.
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Compartmentalization of metabolic pathways in yeast mitochondria improves the production of branched-chain alcohols.
TL;DR: In this article, the Ehrlich pathway was shown to increase the production of isobutanol in Saccharomyces cerevisiae, compared with overexpression of the enzymes involved in the same pathways in the cytoplasm.
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Crystal Structure of the Eukaryotic Strong Inward-Rectifier K+ Channel Kir2.2 at 3.1 Å Resolution
TL;DR: The combination of observations of conductive and inhibitory ion binding sites with electrophysiological data finally explains the mechanism of action of these long-studied channels and reveals how they maintain their low sensitivity to toxins, as well as provides a basis for the design of therapeutic drugs.
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Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds.
Dan Bracha,Mackenzie T. Walls,Ming-Tzo Wei,Lian Zhu,Martin Kurian,José L. Avalos,Jared E. Toettcher,Clifford P. Brangwynne,Clifford P. Brangwynne +8 more
TL;DR: Surprisingly, both experiments and simulations show that while intracellular concentrations may be insufficient for global phase separation, sequestering protein ligands to slowly diffusing nucleation centers can move the cell into a different region of the phase diagram, resulting in localized phase separation.