Joseph A. Sorge

TL;DR: A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed in this article, which eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by restriction digestion and ligation.

TL;DR: These studies demonstrate that the error rate per nucleotide induced in the 182 known detectable sites of the lacI gene was 1.6 x 10(-6) for Pfu DNA polymerase, a greater than tenfold improvement over the 2.0 x 10 (-5) error rate for Taq DNA polymerases.

TL;DR: Pfu formulations with dUTPase exhibited significantly higher efficiencies than Taq, Pfu, and other archaeal DNA polymerases when amplicon length or GC content was increased, according to real-time quantitative PCR data collected during the exponential phase of PCR.

TL;DR: Present rescue efficiencies of greater than 2000 pfu/copy/micrograms of genomic DNA represent a 100,000-fold improvement over initial Rescue efficiencies, permitting quantitative mutational analysis.

TL;DR: The chapter provides information about the modified λ ZAP vector system for the expression of antibody variable regions cloned via polymerase chain reaction (PCR) amplification and the introduction of a modifiedλ Zap vector into the chromosome of transgenic mice for the purpose of generating a λ shuttle vector system capable of measuring spontaneous and induced tissue specific mutation rates in whole animals.