J
Joshua R. Sanes
Researcher at Harvard University
Publications - 399
Citations - 77837
Joshua R. Sanes is an academic researcher from Harvard University. The author has contributed to research in topics: Neuromuscular junction & Postsynaptic potential. The author has an hindex of 138, co-authored 388 publications receiving 71268 citations. Previous affiliations of Joshua R. Sanes include University of Washington & Washington University in St. Louis.
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Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
Evan Z. Macosko,Evan Z. Macosko,Anindita Basu,Anindita Basu,Rahul Satija,Rahul Satija,James Nemesh,James Nemesh,Karthik Shekhar,Melissa Goldman,Melissa Goldman,Itay Tirosh,Allison R. Bialas,Nolan Kamitaki,Nolan Kamitaki,Emily M. Martersteck,John J. Trombetta,David A. Weitz,Joshua R. Sanes,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Aviv Regev,Aviv Regev,Aviv Regev,Steven A. McCarroll,Steven A. McCarroll +26 more
TL;DR: Drop-seq will accelerate biological discovery by enabling routine transcriptional profiling at single-cell resolution by separating them into nanoliter-sized aqueous droplets, associating a different barcode with each cell's RNAs, and sequencing them all together.
Highly Parallel Genome-wide Expression Profiling of Individual Cells Using Nanoliter Droplets
Evan Z. Macosko,Evan Z. Macosko,Anindita Basu,Anindita Basu,Rahul Satija,Rahul Satija,James Nemesh,James Nemesh,Karthik Shekhar,Melissa Goldman,Melissa Goldman,Itay Tirosh,Allison R. Bialas,Nolan Kamitaki,Nolan Kamitaki,Emily M. Martersteck,John J. Trombetta,David A. Weitz,Joshua R. Sanes,Alex K. Shalek,Alex K. Shalek,Alex K. Shalek,Aviv Regev,Aviv Regev,Aviv Regev,Steven A. McCarroll,Steven A. McCarroll +26 more
TL;DR: Drop-seq as discussed by the authors analyzes mRNA transcripts from thousands of individual cells simultaneously while remembering transcripts' cell of origin, and identifies 39 transcriptionally distinct cell populations, creating a molecular atlas of gene expression for known retinal cell classes and novel candidate cell subtypes.
Journal ArticleDOI
Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.
Guoping Feng,Rebecca H. Mellor,Michael L. Bernstein,Cynthia Keller-Peck,Quyen T. Nguyen,Mia Wallace,Jeanne M. Nerbonne,Jeff W. Lichtman,Joshua R. Sanes +8 more
TL;DR: Each of 25 independently generated transgenic lines expressed XFP in a unique pattern, even though all incorporated identical regulatory elements (from the thyl gene), for example, all retinal ganglion cells or many cortical neurons were XFP positive in some lines, whereas only a few ganglions or only layer 5 cortical pyramids were labeled in others.
Neurotechnique Imaging Neuronal Subsets in Transgenic Mice Expressing Multiple Spectral Variants of GFP
Guoping Feng,Rebecca H. Mellor,Michael L. Bernstein,Cynthia Keller-Peck,Quyen T. Nguyen,Mia Wallace,Jeanne M. Nerbonne,Jeff W. Lichtman,Joshua R. Sanes +8 more
Journal ArticleDOI
Long-term in vivo imaging of experience-dependent synaptic plasticity in adult cortex
Joshua T. Trachtenberg,Brian E. Chen,Graham Knott,Guoping Feng,Joshua R. Sanes,Egbert Welker,Karel Svoboda +6 more
TL;DR: The measurements suggest that sensory experience drives the formation and elimination of synapses and that these changes might underlie adaptive remodelling of neural circuits.