J
Juan Llopis
Researcher at University of Granada
Publications - 179
Citations - 11486
Juan Llopis is an academic researcher from University of Granada. The author has contributed to research in topics: Population & Chemistry. The author has an hindex of 36, co-authored 164 publications receiving 10659 citations. Previous affiliations of Juan Llopis include University of Valencia & University of California, San Diego.
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Journal ArticleDOI
Fluorescent indicators for Ca2+based on green fluorescent proteins and calmodulin
Atsushi Miyawaki,Juan Llopis,Roger Heim,J. Michael McCaffery,Joseph A. Adams,Mitsuhiko Ikura,Mitsuhiko Ikura,Roger Y. Tsien +7 more
TL;DR: New fluorescent indicators for Ca2+ that are genetically encoded without cofactors and are targetable to specific intracellular locations are constructed and dubbed ‘cameleons’.
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Measurement of cytosolic, mitochondrial, and Golgi pH in single living cells with green fluorescent proteins.
TL;DR: The pH of the medial/trans-Golgi was measured and demonstrated that the Golgi membrane in intact cells is relatively permeable to H+, and that Cl- serves as a counter-ion for H+ transport and likely helps to maintain electroneutrality.
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New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications.
Stephen R. Adams,Robert E. Campbell,Larry A. Gross,Brent R. Martin,Grant K. Walkup,Yong Yao,Juan Llopis,Roger Y. Tsien +7 more
TL;DR: Affinities in vitro and detection limits in living cells are optimized with Xaa-Xaa = Pro-Gly, suggesting that the preferred peptide conformation is a hairpin rather than the previously proposed alpha-helix.
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Cell-permeant caged InsP3 ester shows that Ca2+ spike frequency can optimize gene expression
TL;DR: Oscillations in cytosolic free calcium levels at roughly physiological rates maximize gene expression for a given amount of InsP3, and this derivative diffused into intact cells and was hydrolysed to produce a caged, metabolically stable InsP 3 derivative.
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Changes in intramitochondrial and cytosolic pH: early events that modulate caspase activation during apoptosis.
Shigemi Matsuyama,Juan Llopis,Juan Llopis,Quinn Deveraux,Roger Y. Tsien,Roger Y. Tsien,John C. Reed +6 more
TL;DR: It is shown that mitochondria-mediated alteration of intracellular pH may be an early event that regulates caspase activation in the mitochondrial pathway for apoptosis, and this finding is supported by results obtained from cells using protonophores.