Julian Gannon

TL;DR: It is concluded that overexpression of p53 is synonymous with mutation, but some mutations would not be detected by a simple immunohistochemical analysis.

TL;DR: It is proposed that immunoprecipitation of p53 by PAb240 is diagnostic of mutation in both murine and human systems and suggested that the different point mutations which convert p53 from a recessive to a dominant oncogene exert a common conformational effect on the protein.

TL;DR: It is shown that the carboxy-terminal region of BRCA2, which interacts directly with the essential recombination protein RAD51, contains a site that is phosphorylated by cyclin-dependent kinases, indicating that S3291 phosphorylation might provide a molecular switch to regulate RAD51 recombination activity.

TL;DR: Microinjection of antibodies against subunits of the anaphase-promoting complex/cyclosome (APC/C) or against human Cdc20 (fizzy) arrested cells at metaphase and stabilized both cyclins A and B1 suggest that the APC/ C is activated as cells enter mitosis and immediately targets cyclin A for degradation, whereas the spindle assembly checkpoint delays the degradation of cyclin B1 until the metaphase to anaphases transition.

TL;DR: Observations suggest that cyclin B1 may compensate for the loss ofcyclin B2 in the mutant mice, and implies that cycl in B1 is capable of targeting the p34(cdc2) kinase to the essential substrates of cyclinB2.