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K.K. Kartha

Bio: K.K. Kartha is an academic researcher from National Research Council. The author has contributed to research in topics: Shoot & Explant culture. The author has an hindex of 2, co-authored 2 publications receiving 91 citations.
Topics: Shoot, Explant culture, Auxin, Phaseolus, Cytokinin

Papers
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Journal ArticleDOI
TL;DR: Analysis of four isozymes, esterase, GDH, 6-PGD, and LAP, as well as nine genetically defined morphological characters indicated retention of genetic stability in the progeny of tisssue culture propagules.

67 citations

Journal ArticleDOI
TL;DR: In vitro morphogenetic responses of shoot apical meristems of 3 species of Phaseolus were studied under various temperature regimes as well as hormone combinations, and Sucrose alone, at 5 % level, appeared to be adequate to bring about growth reduction and thus facilitate short-term preservation ofphaseolusMeristems.

26 citations


Cited by
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Journal ArticleDOI
TL;DR: A reproducible transformation system was developed for pea using as explants sections from the embryonic axis of immature seeds, and transformed plants were resistant to the herbicide Basta when sprayed at rates used in field practice.
Abstract: A reproducible transformation system was developed for pea (Pisum sativum L.) using as explants sections from the embryonic axis of immature seeds. A construct containing two chimeric genes, nopaline synthase-phosphinothricin acetyl transferase (bar) and cauliflower mosaic virus 35S-neomycin phosphotransferase (nptII), was introduced into two pea cultivars using Agrobacterium tumefaciens-mediated transformation procedures. Regeneration was via organogenesis, and transformed plants were selected on medium containing 15 mg/L of phosphinothricin. Transgenic peas were raised in the glasshouse to produce flowers and viable seeds. The bar and nptII genes were expressed in both the primary transgenic pea plants and in the next generation progeny, in which they showed a typical 3:1 Mendelian inheritance pattern. Transformation of regenerated plants was confirmed by assays for neomycin phosphotransferase and phosphinothricin acetyl transferase activity and by northern blot analyses. Transformed plants were resistant to the herbicide Basta when sprayed at rates used in field practice.

253 citations

Journal ArticleDOI
TL;DR: The introduced bar gene has been shown to confer strong resistance in transgenic beans to basta herbicide application in the greenhouse.
Abstract: Transgenic Phaseolus vulgaris or common bean has been produced using electric-discharge particle acceleration. The method uses particle acceleration to introduce DNA into bean seed meristems. Multiple shoots are then generated and screened to recover transgenic plants at a rate of 0.03% germline transformed plants/shoot. We have been able to recover transgenic plants using both GUS and herbicide screening to introduce the gus, bar, and bean golden mosaic virus coat protein genes into the navy bean cultivar, Seafarer. The transgenic plants have been characterized over 5 generations of self-fertilization with no loss of introduced genes or expression. In addition, several families have been crossed with non-transgenic parents and these plants also show expected inheritance patterns. The introduced bar gene has been shown to confer strong resistance in transgenic beans to basta herbicide application in the greenhouse.

157 citations

Book
11 Dec 2000
TL;DR: Chemistry nutrition plant physiology, agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates, and research into breeding strategies to improve grain legumes carbohydrates are presented.
Abstract: Chemistry nutrition plant physiology and agronomy processing biotechnology breeding strategies for improving grain legume carbohydrates. (Part contents).

110 citations

Journal ArticleDOI
TL;DR: Levels of regeneration were increased by having abaxial rather than adaxial surface in contact with the regeneration medium, although this effect was dependent on the presence of 0.1 or 1.0 mM putrescine but not the DNA methylation inhibitor 5-azacytidine.

105 citations

Journal ArticleDOI
TL;DR: The efficacy of benzyladenine to induce multiple shoots from seed explants of chickpea was assessed and 80% of the regenerants developed into morphologically normal and fertile plants.
Abstract: The efficacy of benzyladenine (BA) to induce multiple shoots from seed explants of chickpea (Cicer arietinum L.) was assessed. Shoot differentiation was influenced by the type of seed explant, genotype and concentration of BA. Orientation of the explant also strongly influenced the shoot regeneration response. The optimum BA concentration for shoot/shoot bud regeneration was genotype dependent. Two types of BA-induced response were observed: (1) at less than 7.5 gm BA, direct shoot differentiation (2 to 4-cm-long shoots) was observed within 30 days; (2) at higher BA concentrations (75–100 μm), shoot/shoot bud differentiation was achieved in 45–90 days. A high BA concentration inhibited subsequent rooting of shoots. Roots, however, could be easily induced on shoots derived from <12.5 μm BA. Following transfer to soil, 80% of the regenerants developed into morphologically normal and fertile plants.

92 citations