K
Kaiqin Lao
Researcher at Applied Biosystems
Publications - 66
Citations - 18136
Kaiqin Lao is an academic researcher from Applied Biosystems. The author has contributed to research in topics: microRNA & Gene expression profiling. The author has an hindex of 37, co-authored 66 publications receiving 16361 citations. Previous affiliations of Kaiqin Lao include University of California, Berkeley & Stanford University.
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Journal ArticleDOI
Real-time quantification of microRNAs by stem–loop RT–PCR
Caifu Chen,Dana Ridzon,Adam Broomer,Zhaohui Zhou,Danny H. Lee,Julie T. Nguyen,Maura Barbisin,Nan Lan Xu,Vikram R. Mahuvakar,Mark R. Andersen,Kaiqin Lao,Kenneth J. Livak,Karl J. Guegler +12 more
TL;DR: A novel microRNA quantification method has been developed using stem–loop RT followed by TaqMan PCR analysis, which enables fast, accurate and sensitive miRNA expression profiling and can identify and monitor potential biomarkers specific to tissues or diseases.
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mRNA-Seq whole-transcriptome analysis of a single cell.
Fuchou Tang,Catalin Barbacioru,Yangzhou Wang,Ellen Nordman,Clarence Lee,Nanlan Xu,Xiaohui Wang,John Bodeau,Brian B. Tuch,Asim Siddiqui,Kaiqin Lao,M. Azim Surani +11 more
TL;DR: A single-cell digital gene expression profiling assay with only a single mouse blastomere is described, which detected the expression of 75% more genes than microarray techniques and identified 1,753 previously unknown splice junctions called by at least 5 reads.
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Single-cell RNA-Seq profiling of human preimplantation embryos and embryonic stem cells
Liying Yan,Mingyu Yang,Hongshan Guo,Lu Yang,Jun Wu,Rong Li,Rong Li,Ping Liu,Ying Lian,Xiaoying Zheng,Jie Yan,Jin Huang,Ming Li,Xinglong Wu,Lu Wen,Kaiqin Lao,Ruiqiang Li,Jie Qiao,Jie Qiao,Fuchou Tang +19 more
TL;DR: It is found that EPI cells and primary hESC outgrowth have dramatically different transcriptomes, with 1,498 genes showing differential expression between them, and this work provides a comprehensive framework of the transcriptome landscapes of human early embryos and hESCs.
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Downregulation of miRNA-200c Links Breast Cancer Stem Cells with Normal Stem Cells
Yohei Shimono,Maider Zabala,Robert W. Cho,Neethan A. Lobo,Piero Dalerba,Dalong Qian,Maximilian Diehn,Huiping Liu,Sarita Panula,Eric Chiao,Frederick M. Dirbas,George Somlo,Renee A. Reijo Pera,Kaiqin Lao,Michael F. Clarke +14 more
TL;DR: The coordinated downregulation of three microRNA clusters and the similar functional regulation of clonal expansion by miR-200c provide a molecular link that connects BCSCs with normal stem cells.
Journal ArticleDOI
Probing Gene Expression in Live Cells, One Protein Molecule at a Time
TL;DR: It is found that the protein molecules are produced in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule, and that protein copy numbers in the bursts follow a geometric distribution.