Kazutaka Yamada

TL;DR: This work focused on the redox properties of some commercially available fluorescent dyes, and investigated dye-nucleotide interactions between a free dye and a nucleotide in aqueous solution by electrochemical and spectroscopic techniques to estimate the fluorescence quenching intensity.

TL;DR: A simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine.

TL;DR: In this paper, a method for determining a concentration of a target nucleic acid by using a probe labeled with a fluorescent dye is presented, which is capable of reducing fluorescence emission from the fluorescent dye when hybridized with the target n-acid.

TL;DR: In this article, a method for assaying a nucleic acid comprising the steps of hybridizing the probe consisting of a fluorochrome, 2-O-methyl-oligonucleotide, chimeric oligonucleotide mediated by them to a target n-acid, and so on or assaying the decrease in the fluorescence of the fluoroxide before and after the hybridization, the real-time quantitative PCR method using the method, and a method of analyzing data having a process in which a fluorescence intensity value in the annealing reaction is corrected by that

TL;DR: This work applied the PAI-PCR method to isolate glycosyl hydrolase genes from DNAs extracted from vermiform appendixes of horses and termite guts and amplified and cloned successfully the flanking sequences of the target genes, whereas standard IPCR resulted in no amplification.