Kevin E. Ashelford

TL;DR: The method is implemented as a program with a simple-to-use graphic user interface that is capable of running on a range of computer platforms and concludes that, as a conservative estimate, 1 in every 20 public database records is likely to be corrupt.

TL;DR: A new computer program, called Mallard, is presented for screening entire 16S rRNA gene libraries of up to 1,000 sequences for chimeras and other artifacts, which far exceed previous estimates of artifacts within public repositories and highlight the urgent need for all researchers to adequately screen their libraries prior to submission.

TL;DR: PRIMROSE is a computer program for identifying 16S rRNA probes and PCR primers for use as phylogenetic and ecological tools in the identification and enumeration of bacteria and a comparison with PROBE DESIGN within the ARB software package shows that PRIMRose is capable of identifying oligonucleotides with a higher specificity.

TL;DR: It is shown that substantial populations of these viruses exist in soil at least 350-fold more than the highest numbers estimated from traditional viable plaque counts and are likely to be important in controlling bacterial populations and mediating gene transfer in soil.

TL;DR: Pyrosequencing has provided extensive genomic information for M. galloprovincialis and generated novel observations on expression of different tissues, mitochondria and associated microorganisms.