Kimiko Kanehashi

TL;DR: It is shown that KiSS-1 encodes a carboxy-terminally amidated peptide with 54 amino-acid residues, which is isolated from human placenta as the endogenous ligand of an orphan G-protein-coupled receptor (hOT7T175) and named ‘metastin’.

TL;DR: G Protein-Coupled Receptor proteins originating in the vicinity of rat brain stem and human brain or salts thereof, or peptide fragments thereof or amides, esters or salts of the same; ligands thereto; a method/kit for screening compounds capable of altering the binding properties of the ligands to the G protein-coupled receptor proteins as mentioned in this paper.

TL;DR: G Protein-Coupled Receptor proteins originating in the vicinity of rat brain stem and human brain or salts thereof, or peptide fragments thereof or amides, esters or salts of the same; ligands thereto; a method/kit for screening compounds capable of altering the binding properties of the ligands to the G protein-coupled receptor proteins as discussed by the authors.

TL;DR: In this article, a G protein-coupled receptor protein containing a specific amino acid sequence was obtained by cloning a human brain cDNA library using a probe containing a labeled partial base sequence, transducing the resultant gene by means of electroporation to get a transformant, subcloning this transformant with a probe and a primer and expressing the resultant cDNA sequence by the use of an expression system.

TL;DR: The authors concerne des proteines receptrices couplees aux proteines G tirees du voisinage du tronc cerebral du rat and du cerveau humain.