Showing papers by "Kirpal S. Bisht published in 2003"
TL;DR: In this paper, the authors used matrix assisted laser desorption/ionization (MALDI), hyphenated thermogravimetric/mass spectrometry (TG/MS), and dielectric analysis (DEA) for the analysis of polymers damaged by ionizing radiation.
34 citations
TL;DR: It was confirmed from the detailed spectral analysis of the products that transesterification failed to furnish any free hydroxyls on the sophorose ring, and it was proposed that in absence of the lactonic structural motif, the binding of the peracylated non-lactonic SLs in the lipase binding pocket takes place such that the carboxyl group of the octadecenoic acid is preferentially accessible to the active site.
30 citations
TL;DR: Pseudomonas cepacia lipase (PS-30) was used in hydrolytic resolution of 3-acetoxy-4-aryl-substituted azetidin-2-ones and absolute stereochemistry of the enantiomers was established using single crystal X-ray crystallographic techniques.
25 citations
TL;DR: In this paper, the binding of the peracylated non-lactonic SLs in the lipase binding pocket takes place such that the carboxyl group of the octadecenoic acid, not the sophorose sugar, is preferentially accessible to the active site.
Abstract: Regioselective transesterifications and hydrolysis of peracylated sophorolipid (SL) derivatives catalyzed by lipases was investigated. This study is the first evaluation of the lipase-catalyzed reactions on the non-lactonic SL derivatives. Four lipases, namely from porcine pancreas (PPL, Type II), Candida rugosa (AYS, TypeVII), Pseudomonas cepacia (PS-30), and Candida antarctica (Novozym 435, carrier fixed lipase fraction B) were used in anhydrous THF or in phosphate buffer (pH=7.4, 0.2 M). It was confirmed from the detailed spectral analysis of the products that transesterification failed to furnish any free hydroxyls on the sophorose ring. Instead, transesterification took place on the methyl ester located at the carboxylic end of the 17-hydroxyoctadecenoic acid chain attached to the C-1′ position of the sophorose ring. It is proposed that in absence of the lactonic structural motif, the binding of the peracylated non-lactonic SLs in the lipase binding pocket takes place such that the carboxyl group of the octadecenoic acid, not the sophorose sugar, is preferentially accessible to the active site.