Kiwamu Hyodo

TL;DR: This study used a model virus and found that host heat shock proteins Hsp70 and Hsp90 are required for RCNMV RNA replication and that they interact with p27, a virus-encoded component of the 480-kDa replicase complex, on the endoplasmic reticulum membrane.

TL;DR: It is shown that the replication of red clover necrotic mosaic virus (RCNMV), a plant positive-strand RNA [(+)RNA] virus, requires NbRBOHB-mediated ROS production, which is essential for robust viral RNA replication.

TL;DR: Two novel (-)ssRNA mycoviruses in the shiitake mushroom (Lentinula edodes) are discovered and enhance the understanding of the diversity, evolution and spread of (-)SSRNA viruses in fungi.

TL;DR: It is found that inhibition of the nucleotide exchange activity of Arf1 using the inhibitor brefeldin A (BFA) disrupted the assembly of the viral replicase complex and p27-mediated ER remodeling, suggesting that the replication of RCNMV depends on the host membrane traffic machinery.

TL;DR: Virus-induced gene silencing of NbGAPDH-A reduced RCNMV multiplication in the inoculated leaves, but not in the single cells, thereby suggesting that GAPDH, a component of the Calvin-Benson cycle in chloroplasts, plays a positive role in cell-to-cell movement of RC NMV.