Author
Klaus Weber
Other affiliations: Cold Spring Harbor Laboratory
Bio: Klaus Weber is an academic researcher from Harvard University. The author has contributed to research in topics: RNA & Peptide sequence. The author has an hindex of 23, co-authored 30 publications receiving 23041 citations. Previous affiliations of Klaus Weber include Cold Spring Harbor Laboratory.
Topics: RNA, Peptide sequence, Cistron, Sodium dodecyl sulfate, Q beta Replicase
Papers
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TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
19,381 citations
TL;DR: In this modification of the 5-dimethylaminonaphthalene-1-sulfonyl-Edman degradation, inorganic carriers permit convenient manipulation of small amounts of protein, and use of the detergent sodium dodecyl sulfate throughout the procedure maintains protein solubility.
603 citations
01 Jan 1975
556 citations
TL;DR: Successful renaturation of several oligomeric enzymes after denaturation by sodium dodecyl sulfate is described and the results obtained suggest a general procedure for reactivation of enzymes after treatment with this detergent.
411 citations
TL;DR: The unusual guanosine nucleotides, MSI and MSII, accumulated in vivo during amino-acid starvation of stringent strains of E. coli are shown to be synthesized in vitro as a product of an idling step in protein synthesis occurring on the ribosomes.
Abstract: The unusual guanosine nucleotides, MSI and MSII, accumulated in vivo during amino-acid starvation of stringent strains of E. coli are shown to be synthesized in vitro as a product of an idling step in protein synthesis occurring on the ribosomes.
299 citations
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TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Abstract: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
232,912 citations
Journal Article•
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Abstract: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products. Four major components of the head are cleaved during the process of assembly, apparently after the precursor proteins have assembled into some large intermediate structure.
203,017 citations
TL;DR: The results show that the polyacrylamide gel electrophoresis method can be used with great confidence to determine the molecular weights of polypeptide chains for a wide variety of proteins.
19,381 citations
TL;DR: The purification of homogeneous glutathione S-transferases B and C from rat liver is described, and only transferases A and C are immunologically related.
16,953 citations
TL;DR: A rapid and convenient method for peptide mapping of proteins has been developed that involves partial enzymatic proteolysis in the presence of sodium dodecyl sulfate and analysis of the cleavage products by polyacrylamide gel electrophoresis.
4,933 citations