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Showing papers by "Kodiveri Muthukaliannan Gothandam published in 2017"



Journal ArticleDOI
TL;DR: Both the RNAi and antisense lines showed a significant decrease in the expression levels of phytoene desaturase and carotenoid content compared to wild type cells.

29 citations


Journal ArticleDOI
TL;DR: Dunaliella salina lyophilized powder was found to show a chemopreventive effect against breast cancer induced in rats through the suppression of cell proliferation and the induction of apoptosis.
Abstract: Dunaliella salina is a photosynthetic cell factory used for the commercial production of food additives, cattle stock feed and cosmetics as well as active ingredients for pharmaceutical industries. The investigation of the in vivo antitumor activity of D. salina lyophilized powder (DSLP) against 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Wistar rats indicated a dose-dependent effect of DSLP. We studied the effect of DSLP at two different dosages of 500 and 1000 mg per kg bw on DMBA induced mammary cancer in rats by measuring the status of antioxidant enzymes, phase I and phase II detoxification enzymes, lipid peroxidation, and glycoconjugated proteins and by investigating the expression pattern of cell proliferation (Ki-67), hormonal receptor (ER, PR and HER2) status by immunohistochemical analysis, and apoptotic (caspase-3 and -9) and pro-inflammatory (COX-2) markers by colorimetric analysis. After 16 weeks of the study, we observed 100% tumor formation (including high tumor incidence and tumor volume) and a significant increase in the level of hormonal receptors, cell proliferation, and pro-inflammatory and apoptosis markers in tumor-bearing animals compared to the control. The oral administration of DSLP (1000 mg per kg bw) to the DMBA treated animals showed up to 83.4% reduction of tumors and effectively restored the levels of biochemical markers in the mammary tissues in addition to the downregulation of the expression of molecular markers. In conclusion, DSLP was found to show a chemopreventive effect against breast cancer induced in rats through the suppression of cell proliferation and the induction of apoptosis.

24 citations


Journal ArticleDOI
TL;DR: This study indicates the emergence of colistin resistant isolates from clinical samples among different groups of gram-negative organisms and develops new antibiotics and programs to reduce nosocomial infections.
Abstract: Background: Colistin is one of the oldest antibiotics in the polymyxin group, and is used mostly against gramnegative bacteria. Because of developing resistance among clinical isolates colistin has become an alternative drug for multidrug resistant bacteria. Objectives: To determine colistin resistance among isolates from Tamil Nadu, India. Methods: We included 94 gram-negative isolates from two centers in Tamil Nadu in the present study. Isolates were identified by 16S rRNA sequencing. Minimal inhibitory concentrations (MICs) were determined by agar dilution. Results: The isolates identified at species level included 48 Escherichia coli, 9 Klebsiella pneumoniae, 10 Pseudomonas aeruginosa, 5 Proteus mirabilis, 4 Salmonella enterica, 3 Enterobacter hormaechei, 3 Enterobacter cloacae, 2 Achromobacter xylosoxidans, 2 Acinetobacter baumannii, 1 Providencia vermicola, 1 Acinetobacter towneri, 1 Enterobacter gergoviae, 2 Providencia rettgeri, 1 Enterobacter asburiae, 1 Pseudomonas stutzeri, and 1 Salmonella typhi. The MIC of colistin ranged from 0.12 ?g/ml to 128 ?g/ml. The MIC50 was 1 ?g/mL and MIC90 was >128 ?g/ml. The MIC ? 8 ?g/mL was resistant breakpoint for all the species. A total of 27 isolates were resistant to colistin. Colistin resistant isolates included E. coli (9/48), K. pneumoniae (6/9), P. aeruginosa (3/10), A. baumannii (1/2), P. mirabilis (4/5), E. cloacae (1/3), P. rettgeri (2/2), and S. enterica (1/4). Carbapenem susceptibility of colistin resistant isolates was tested and 14 were found to be resistant to meropenem. Conclusions: Our study indicates the emergence of colistin resistant isolates from clinical samples among different groups of gram-negative organisms. Resistance to both carbapenem and colistin occurs. Developing new antibiotics and programs to reduce nosocomial infections is necessary especially for multidrug resistant isolates. Keywords: Antimicrobial agents, gram negative, multi-drug resistant, pathogenesis, polymyxin

17 citations


01 Jul 2017
TL;DR: This paper presents a meta-modelling procedure called “Smart grids” (Smart grids) for smart grids that solve the challenge of integrating smart grids to solve the problem of drought in rural areas.
Abstract: Ramachandran Srinivasan 1,† , Anbazhagan Mageswari 2,† , Parthiban Subramanian 3 , Vaibhav Kumar Maurya 1 , Chandrasekaran Sugnathi 1 , Chaitanyakumar Amballa 1 , Tongmin Sa 4 & Gothandam KM 1 * School of Bio-Sciences and Technology, VIT University, Vellore-632 014, Tamil Nadu, India Agricultural Microbiology Division, Department of Agricultural Biology; Department of Agricultural Biotechnology (Metabolic Engineering Division), National Institute of Agricultural Sciences, Rural Development Administration, Jeonju, Republic of Korea Department of Environmental and Biological Chemistry, Chungbuk National University, Cheongju, Chungbuk 361-763, Republic of Korea

15 citations