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Kodiveri Muthukaliannan Gothandam

Bio: Kodiveri Muthukaliannan Gothandam is an academic researcher from VIT University. The author has contributed to research in topics: Gene & Publication bias. The author has an hindex of 19, co-authored 78 publications receiving 1108 citations. Previous affiliations of Kodiveri Muthukaliannan Gothandam include Chungbuk National University & C. Abdul Hakeem College.


Papers
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Journal ArticleDOI
TL;DR: The results suggest that the OsPPR1 is a nuclear gene of rice, encoding the PPR protein that might play a role in the chloroplast biogenesis.
Abstract: In this paper, we report a novel pentatricopeptide repeat (PPR) protein gene in rice. PPR, a characteristic repeat motif consisted of tandem 35 amino acids, has been found in various biological systems including plant. Sequence analysis revealed that the gene designated OsPPR1 consisted of an open reading frame of 2433 nucleotides encoding 810 amino acids that include 11 PPR motifs. Blast search result indicated that the gene did not align with any of the characterized PPR genes in plant. The OsPPR1 gene was found to contain a putative chloroplast transit peptide in the N-terminal region, suggesting that the gene product targets to the chloroplast. Southern blot hybridization indicated that the OsPPR1 is the member of a gene family within the rice genome. Expression analysis and immunoblot analysis suggested that the OsPPR1 was accumulated mainly in rice leaf. Antisense transgenic strategy was used to suppress the expression of OsPPR1 and the resulted transgenic rice showed the typical phenotypes of chlorophyll-deficient mutants; albinism and lethality. Cytological observation using microscopy revealed that the antisense transgenic plant contained a significant defect in the chloroplast development. Taken together, the results suggest that the OsPPR1 is a nuclear gene of rice, encoding the PPR protein that might play a role in the chloroplast biogenesis. This is the first report on the PPR protein required for the chloroplast biogenesis in rice.

115 citations

Journal Article
TL;DR: Results showed that leaf area and dry matter content of tomato fruits decreased with application of elevated salt stress, however endogenous content of IAA, ABA and proline was found to be increasing with increase in salt treatment, suggesting that leaves are more sensitive than fruits.
Abstract: Tomato cultivar PKM 1 were subjected to 25, 50, 100, 150 and 200 mM NaCl stress and response of tomato plant to saltstress were determined by assessing the variability of different biochemical parameters In this present study endogenouscontent of growth hormones IAA and ABA in leaves, proline and mineral (Na+ and K+) content in leaves and maturefruits were estimated Leaf area and dry matter content of tomato fruits under salt stress were determined to study theeffect of salinity on photosynthetic yield Results showed that leaf area and dry matter content of tomato fruits decreasedwith application of elevated salt stress, however endogenous content of IAA, ABA and proline was found to beincreasing with increase in salt treatment Application of NaCl caused increase in Na+ content, while K+ content andK+/Na+ ratio decreased with increase in salt stress Another striking point is that increase in proline and Na+ contentwas more in leaves than fruits, which suggests that leaves are more sensitive than fruitsKeyword: Salt stress, IAA, ABA, Proline, Na+, dry matter

81 citations

Journal ArticleDOI
01 Jun 2019-Medicine
TL;DR: This meta-analysis protocol examines the comparative utility of neutrophil-lymphocyte-ratio, platelet-ly mphocyte- ratio, and monocyte-lyymphocyte-Ratio as a simple, inexpensive, and effective method for cancer prognosis, in multiple cancers.

72 citations

Journal ArticleDOI
TL;DR: Studies on the effect of different carbon and nitrogen sources revealed that xylose and urea enhances the enzyme production, and with selected C–N sources along with 1 M NaCl the maximum protease production was obtained.

67 citations

Journal ArticleDOI
TL;DR: The findings suggested that PhCsNPs augmented the mitochondrial-mediated apoptotic mechanism through the stimulation of oxidative stress, depletion of cellular antioxidants and cell cycle arrest, and could be used as an efficient therapeutic agent for the treatment of oral cancer.

61 citations


Cited by
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01 Jun 2012
TL;DR: SPAdes as mentioned in this paper is a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler and on popular assemblers Velvet and SoapDeNovo (for multicell data).
Abstract: The lion's share of bacteria in various environments cannot be cloned in the laboratory and thus cannot be sequenced using existing technologies. A major goal of single-cell genomics is to complement gene-centric metagenomic data with whole-genome assemblies of uncultivated organisms. Assembly of single-cell data is challenging because of highly non-uniform read coverage as well as elevated levels of sequencing errors and chimeric reads. We describe SPAdes, a new assembler for both single-cell and standard (multicell) assembly, and demonstrate that it improves on the recently released E+V-SC assembler (specialized for single-cell data) and on popular assemblers Velvet and SoapDeNovo (for multicell data). SPAdes generates single-cell assemblies, providing information about genomes of uncultivatable bacteria that vastly exceeds what may be obtained via traditional metagenomics studies. SPAdes is available online ( http://bioinf.spbau.ru/spades ). It is distributed as open source software.

10,124 citations

01 Jan 2012

3,692 citations

Journal ArticleDOI
TL;DR: Several recent papers are discussed that cover the evolutionary history and molecular mode of action of Pentatricopeptide repeat proteins, and propose hypotheses for their physiological roles that could explain why PPR proteins are so numerous in terrestrial plants.

772 citations

Journal ArticleDOI
TL;DR: It is shown in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide and plays an additional role in promoting the editing of atp 6 mRNAs, independent of its cleavage function.
Abstract: Cytoplasmic male sterility (CMS) and nucleus-controlled fertility restoration are widespread plant reproductive features that provide useful tools to exploit heterosis in crops. However, the molecular mechanism underlying this kind of cytoplasmic-nuclear interaction remains unclear. Here, we show in rice (Oryza sativa) with Boro II cytoplasm that an abnormal mitochondrial open reading frame, orf79, is cotranscribed with a duplicated atp6 (B-atp6) gene and encodes a cytotoxic peptide. Expression of orf79 in CMS lines and transgenic rice plants caused gametophytic male sterility. Immunoblot analysis showed that the ORF79 protein accumulates specifically in microspores. Two fertility restorer genes, Rf1a and Rf1b, were identified at the classical locus Rf-1 as members of a multigene cluster that encode pentatricopeptide repeat proteins. RF1A and RF1B are both targeted to mitochondria and can restore male fertility by blocking ORF79 production via endonucleolytic cleavage (RF1A) or degradation (RF1B) of dicistronic B-atp6/orf79 mRNA. In the presence of both restorers, RF1A was epistatic over RF1B in the mRNA processing. We have also shown that RF1A plays an additional role in promoting the editing of atp6 mRNAs, independent of its cleavage function.

568 citations

Journal ArticleDOI
TL;DR: The cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology are reported, which suggests that the members of a family may have different functions.
Abstract: MicroRNAs (miRNAs), a group of small non-coding RNAs, have recently become the subject of intense study. They are a class of post-transcriptional negative regulators playing vital roles in plant development and growth. However, little is known about their regulatory roles in the responses of trees to the stressful environments incurred over their long-term growth. Here, we report the cloning of small RNAs from abiotic stressed tissues of Populus trichocarpa (Ptc) and the identification of 68 putative miRNA sequences that can be classified into 27 families based on sequence homology. Among them, nine families are novel, increasing the number of the known Ptc-miRNA families from 33 to 42. A total of 346 targets was predicted for the cloned Ptc-miRNAs using penalty scores of

479 citations