K
Krista A. Budinich
Researcher at University of Pennsylvania
Publications - 7
Citations - 144
Krista A. Budinich is an academic researcher from University of Pennsylvania. The author has contributed to research in topics: Myeloid leukemia & Gene. The author has an hindex of 3, co-authored 4 publications receiving 42 citations.
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Journal ArticleDOI
High-performance CRISPR-Cas12a genome editing for combinatorial genetic screening.
Rodrigo A. Gier,Krista A. Budinich,Niklaus H. Evitt,Zhendong Cao,Elizabeth Freilich,Qingzhou Chen,Jun Qi,Yemin Lan,Rahul M. Kohli,Junwei Shi +9 more
TL;DR: The authors engineer AsCas 12a and the crRNA to improve double knockout for synthetic sick/lethal interaction genetic screening and demonstrate the performance of the optimized AsCas12a (opAsCas12A) through double knockout screening against epigenetic regulators.
Journal ArticleDOI
Genetic screening for single-cell variability modulators driving therapy resistance
Eduardo A. Torre,Eri Arai,Sareh Bayatpour,Connie L. Jiang,Lauren E. Beck,Benjamin L. Emert,Sydney M. Shaffer,Ian A. Mellis,Mitchell Fane,Gretchen M. Alicea,Krista A. Budinich,Ashani T. Weeraratna,Junwei Shi,Arjun Raj +13 more
TL;DR: In melanoma, transient fluctuations in the molecular state of tumor cells mark the formation of rare cells primed to survive BRAF inhibition and reprogram into a stably drug-resistant fate as discussed by the authors.
Posted ContentDOI
Genetic screening for single-cell variability modulators driving therapy resistance
Eduardo A. Torre,Eri Arai,Sareh Bayatpour,Lauren E. Beck,Benjamin L. Emert,Sydney M. Shaffer,Ian A. Mellis,Mitchell Fane,Gretchen M. Alicea,Krista A. Budinich,Ashani T. Weeraratna,Junwei Shi,Arjun Raj +12 more
TL;DR: This work used CRISPR/Cas9 genetic screens to reveal genes that affect cell fate decisions by altering cellular plasticity across a range of functional categories and discovered a novel mode of altering resistance that, contrary to known mechanisms, pushes cells towards a more differentiated state.
Journal ArticleDOI
ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency
Zhendong Cao,Krista A. Budinich,Hua Huang,Diqiu Ren,Bin Lu,Zhen Zhang,Qingzhou Chen,Yeqiao Zhou,Yu-Han Huang,Fatemeh Alikarami,Molly C. Kingsley,Alexandra Lenard,Aoi Wakabayashi,Aoi Wakabayashi,Eugene Khandros,Eugene Khandros,Will Bailis,Jun Qi,Martin Carroll,Gerd A. Blobel,Gerd A. Blobel,Robert B. Faryabi,Kathrin M. Bernt,Kathrin M. Bernt,Shelley L. Berger,Junwei Shi +25 more
TL;DR: In this article, the authors discover and characterize the mechanism by which the chromatin "reader" ZMYND8 directly activates IRF8 in parallel with the MYC proto-oncogene through their lineage-specific enhancers.
Journal ArticleDOI
The interferon-stimulated gene RIPK1 regulates cancer cell intrinsic and extrinsic resistance to immune checkpoint blockade.
Lisa Cucolo,Qingzhou Chen,Jingya Qiu,Yong-wei Yu,Max Klapholz,Krista A. Budinich,Zhaojun Zhang,Yue Shao,Igor E. Brodsky,Martha S. Jordan,D. Gary Gilliland,Nan Zhang,Junwei Shi,Andy J. Minn +13 more
TL;DR: Genetic interaction screening revealed that in cancer cells, RIPK1 diverted TNF signaling through NF-κB and away from its role in cell death, which promoted an immunosuppressive chemokine program bycancer cells, enhanced cancer cell survival, and decreased infiltration of T and NK cells expressing TNF superfamily ligands.