Kyle R. Frischkorn

TL;DR: This study sequenced DNA from complex sediment and planktonic consortia from an aquifer adjacent to the Colorado River and reconstructed the first complete genomes for Archaea using cultivation-independent methods, which dramatically expand genomic sampling of the domain Archaea and clarify taxonomic designations within a major superphylum.

TL;DR: The potential roles of Chloroflexi in sediment carbon cycling beyond organohalide respiration are expanded to include respiration of sugars, fermentation, CO2 fixation, and acetogenesis with ATP formation by substrate-level phosphorylation.

TL;DR: The hypothesis that bacteria from phyla lacking cultivated representatives are small and thus might be enriched by filtration for coupled genomic and ultrastructural characterization is tested.

TL;DR: A new version of EMIRGE optimized for large data size is used to reconstruct near-full-length 16S rRNA genes from amplicons sheared and sequenced with Illumina technology, allowing for sensitive, accurate profiling of the “long tail” of low abundance organisms that exist in many microbial communities, and can resolve population dynamics in response to environmental change.

TL;DR: Proteogenomics gives an insight into ecosystem behavior following addition of simple organic carbon to the subsurface, and demonstrates a range of biological processes and community interactions were stimulated.