Author
L.D. Pettit
Bio: L.D. Pettit is an academic researcher from University of Leeds. The author has contributed to research in topics: Potentiometric titration & Determination of equilibrium constants. The author has an hindex of 1, co-authored 1 publications receiving 1149 citations.
Papers
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TL;DR: In this paper, the effect of unknown junction potentials on acid dissociation constants has been investigated, with particular references to nitrilotriacetic acid (NTA) and ethylenediaminetetric acid (EDTA).
1,197 citations
Cited by
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TL;DR: A linear correlation between pH-meter readings in equivalent D2O and H2O solutions, determined experimentally, leads to a novel equation, which allows for a direct recalculation of pKa values measured in D 2O into a H 2O equivalent: pKH=0.929pKH*+0.42.
423 citations
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TL;DR: The potentiometric and spectroscopic data show that acetylation of the amino terminal group induces significant changes in the coordination properties of the (Ac-1-16H) and (1-2-16M) peptides compared to the (1
204 citations
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TL;DR: Results are presented for the purity of three commercial brands of the Ca chelator EGTA as determined with pH-metric and oxalate precipitation methods and other techniques, and the adjustments necessary to allow for ionic strength and the correction of pH readings to yield true [H+] activity or concentration values are described.
Abstract: Results are presented for the purity of three commercial brands of the Ca chelator EGTA [ethyleneglycol-bis(beta-aminoethylether)-N,N'-tetraacetic acid] as determined with pH-metric and oxalate precipitation methods and other techniques. The effect that the observed degree of hydration (2-4%) has on calculated values for [Ca2+] in Ca-EGTA solutions is described with reference to a theoretical dose-response relationship. In addition to the corrections for purity, the adjustments necessary to allow for ionic strength and the correction of pH readings to yield true [H+] activity or concentration values are described. These several corrections result in both a shift and a shape change for the dose-response curve compared with the curve calculated without these corrective procedures. Some authors have already taken some of these aspects into account, but this has not generally been made clear in the literature heretofore. The points raised may help to account for the wide variation of reported [Ca2+] ranges for Ca-sensitive cellular processes.
203 citations
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TL;DR: The quantitative description of these complexes in solution and the solid state provides the basis for predictions of interference from DTT in studies of metal ion binding of thiol-containing biomolecules.
196 citations