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L.F. Gibson

Bio: L.F. Gibson is an academic researcher from University of Technology, Sydney. The author has contributed to research in topics: Aeromonas media & Perna canaliculus. The author has an hindex of 15, co-authored 18 publications receiving 1404 citations.

Papers
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Journal ArticleDOI
TL;DR: This review provides a comprehensive summary of probiotics in aquaculture with special reference to mollusc culture.

756 citations

Journal ArticleDOI
TL;DR: The ability of BLIS-(bacteriocin-like inhibitory substance)-producing Aeromonas media, strain A199, to act as a probiotic was assessed on the host animal, Crassostrea gigas, to prevent death of the oyster larvae when challenged with Vibrio tubiashii.

189 citations

Journal ArticleDOI
TL;DR: Histopathology indicated that the route of infection was via the digestive system and Koch's postulates were confirmed for the two isolates, the first report on pathogens of GSM larvae.
Abstract: Bacterial pathogens of Greenshell mussel (GSM) larvae can cause batch losses during hatchery production. Twenty-two isolates were screened using a larval bioassay. Two strains were identified as potential pathogens. Phenotypic identification of these strains revealed two non-reactive Gram-negative, oxidase positive rods. Sequencing of the 16S rRNA gene identified Vibrio splendidus and a V. coralliilyticus/neptunius-like isolate as pathogens of GSM larvae, with an ability to cause 83% and 75% larval mortality in vitro, respectively, at a concentration of 10(2) CFU mL(-1). Histopathology indicated that the route of infection was via the digestive system. Using healthy larvae as target hosts, Koch's postulates were confirmed for the two isolates. This is the first report on pathogens of GSM larvae.

87 citations

Journal ArticleDOI
TL;DR: It was concluded that A199 is a potential agent for the management of winter outbreaks of saprolegniosis in eels with temperature appearing to be a factor regulating the effect of A199.

81 citations

Journal ArticleDOI
TL;DR: In four independent in vivo tank observations of fish affected with saprolegniosis, the daily addition of A199 to tank water contributed to the subsequent swift recovery of affected hosts from invasion by this opportunistic pathogen.
Abstract: A bacteriocin-like inhibitory substance (BLIS) produced by Aeromonas media strain A199 inhibited the growth of Saprolegnia sp. in vitro, an opportunistic pathogen isolated from affected eels, Anguilla australis (Richardson). The presence of BLIS in solid media inhibited the growth of the vegetative state of the aquatic mould as well as the germination of cysts. Uninhibited growth was, however, observed in the presence of inactive BLIS, suggesting that the in vitro antagonism derived from the BLIS of A199. In four independent in vivo tank observations of fish affected with saprolegniosis, the daily addition of A199 to tank water contributed to the subsequent swift recovery of affected hosts from invasion by this opportunistic pathogen.

52 citations


Cited by
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Journal ArticleDOI
TL;DR: This review describes the state of the art of probiotic research in the culture of fish, crustaceans, mollusks, and live food, with an evaluation of the results obtained so far.
Abstract: There is an urgent need in aquaculture to develop microbial control strategies, since disease outbreaks are recognized as important constraints to aquaculture production and trade and since the development of antibiotic resistance has become a matter of growing concern. One of the alternatives to antimicrobials in disease control could be the use of probiotic bacteria as microbial control agents. This review describes the state of the art of probiotic research in the culture of fish, crustaceans, mollusks, and live food, with an evaluation of the results obtained so far. A new definition of probiotics, also applicable to aquatic environments, is proposed, and a detailed description is given of their possible modes of action, i.e., production of compounds that are inhibitory toward pathogens, competition with harmful microorganisms for nutrients and energy, competition with deleterious species for adhesion sites, enhancement of the immune response of the animal, improvement of water quality, and interaction with phytoplankton. A rationale is proposed for the multistep and multidisciplinary process required for the development of effective and safe probiotics for commercial application in aquaculture. Finally, directions for further research are discussed.

2,072 citations

Journal ArticleDOI
Francois Joel Gatesoupe1
TL;DR: The most promising prospects are sketched out, but considerable efforts of research will be necessary to develop the applications to aquaculture.

1,182 citations

Journal ArticleDOI
TL;DR: This review provides a summary of the use of probiotics for prevention of bacterial diseases in aquaculture, with a critical evaluation of results obtained to date.

1,042 citations

Journal ArticleDOI
S.K. Nayak1
TL;DR: The review is aiming to highlight the immunomodulatory activity of probiotics and also to evaluate the factors that regulate for the optimum induction of immune responses in fish.

994 citations

Book ChapterDOI
30 Apr 2014
TL;DR: The methods described in this chapter are based primarily on methods developed for the characterization and identification of organisms which have usually been isolated on nutrient-rich media.
Abstract: In performing phenotypic characterization tests, the organisms used for the inoculation of test media should be from fresh transfers and in good physiological condition. The methods described in this chapter are based primarily on methods developed for the characterization and identification of organisms which have usually been isolated on nutrient-rich media. While zoologists may be content to classify all insects or all vertebrates, prokaryote systematics generally deals with all prokaryotes and in an evolutionary context attempts to extend this study back to the origin of life itself. To test for acetamide hydrolysis, streak the surface of an acetamide agar slant with a sample from a dilute suspension. Incubate for up to 7 days. Care should be taken to ensure that the basal medium to which the carbohydrates are added does not contain fermentable or oxidizable sugars, and control media without any added carbohydrates should always be included during testing of cultures. Methanogenic members of the Archaea also fluoresce under UV light. Some facultative anaerobes, such as Escherichia coli and Staphylococcus aureus, grow aerobically by respiration with oxygen and grow anaerobically by fermentation.

911 citations