Author
L. L. Salomon
Bio: L. L. Salomon is an academic researcher. The author has contributed to research in topics: Glucose oxidase. The author has an hindex of 1, co-authored 1 publications receiving 123 citations.
Topics: Glucose oxidase
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123 citations
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01 Jan 1965
TL;DR: This chapter discusses an enzymatic method for the determination of D -glucose with hexokinase and glucose-6-phosphate dehydrogenase, a method based on the optical method developed by O. Warburg and his collaborators.
Abstract: Publisher Summary This chapter discusses an enzymatic method for the determination of D -glucose with hexokinase and glucose-6-phosphate dehydrogenase. The optical method for the determination of glucose-6-phosphate, on which the method discussed in the chapter is based, was developed by O. Warburg and his collaborators. Hexokinase catalyzes the phosphorylation of glucose by adenosine triphosphate (ATP). Glucose-6-phosphate is oxidized in the presence of triphosphopyridine nucleotide (TPN) by glucose-6-phosphate dehydrogenase. For all practical purposes, both the reactions proceed stoichiometrically and quantitatively, although reversibility has been demonstrated under special conditions. The reduced triphosphopyridine nucleotide (TPNH) arising in the second reaction is determined spectrophotometrically at 340 or 366 mμ and serves as a measure of the glucose-6-phosphate formed from the glucose in the first reaction. The preparation need not be crystalline but must be relatively free from compounds that interfere with the glucose determination. Sometimes, the preparations still contain glucose, which has been added as a stabilizing agent during purification. It can be removed from highly purified hexokinase by dialysis. The least interference from contaminating enzymes occurs if the specific activity of the hexokinase preparation used approaches that of the crystalline enzyme.
1,022 citations
TL;DR: In this paper, the Enzymatic Determination of Blood Glucose (EDG) was used to determine the level of blood glucose in a human subject with respect to a blood sample.
Abstract: (1960). On the Enzymatic Determination of Blood Glucose. Scandinavian Journal of Clinical and Laboratory Investigation: Vol. 12, No. 4, pp. 402-407.
859 citations
TL;DR: In this paper, the concentration of hydrogen peroxide (H2O2) in distilled water, drinking water and in different types of surface and rain waters can be easily determined by a photometric method in which N,N-diethyl-p-phenylenediamine (DPD) is oxidized by a peroxidase catalyzed reaction.
819 citations
TL;DR: Significant alteration in the kinetics of sugar transport occurs in the infected cells concomitant with changes in cell morphology and these alterations appear to be expressed by the transforming gene(s) of RSV and are not found in cells infected with an avian leukosis virus.
220 citations
TL;DR: A method has been constructed which gives accurate glucose values in whole blood, plasma, and red blood cells which is based on the glucose oxidase method for determination of glucose.
Abstract: A methodological investigation of the glucose oxidase method for determination of glucose is described. The importance of eliminating substances reducing H2O2 generated from glucose and other substances causing unspecific oxidation of the chromogen is emphasized. On the basis of this study a method has been constructed which gives accurate glucose values in whole blood, plasma, and red blood cells.
189 citations