Author
L. Marai
Bio: L. Marai is an academic researcher from University of Toronto. The author has contributed to research in topics: Gas chromatography. The author has an hindex of 1, co-authored 1 publications receiving 34 citations.
Topics: Gas chromatography
Papers
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TL;DR: It is shown that a good gas chromatographic technique effectively counteracts weaknesses in other analytical steps and frequently permit perfect reconstitution of the overall and positional distribution of fatty acids, which must be the ultimate test of the success of the entire analytical scheme.
Abstract: Inclusion of gas chromatography of diglycerides in the various schemes proposed for sub-fractionation of natural glyceryl phosphatides increases the accuracy of identification and quantitation of the individual molecular species. This is due to its efficiency in classifying the molecular weights and proportions of the diglycerides recovered from thin-layer chromatography according to their degree of unsaturation. Determination of the complete structure of glyceryl phosphatides requires standardization of all steps of the analytical system including lipid extractions, enzyme hydrolyses, and thin-layer and gas chromatography. This presentation reviews some of the practical aspects of quantitative gas chromatography of diglycerides and fatty acids as applied in the determination of the molecular species of glyceryl phosphatides. It is shown that a good gas chromatographic technique effectively counteracts weaknesses in other analytical steps. The additional cross-checks provided by the gas chromatography of the diglycerides greatly improve the overall accuracy of the data and frequently permit perfect reconstitution of the overall and positional distribution of fatty acids, which must be the ultimate test of the success of the entire analytical scheme.
34 citations
Cited by
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TL;DR: I-Acyl lysophosphatidylcholine, labelled with [3H]stearate and [14C]glycerol, injected intracerebrally, was incorporated intact into diacyl phosphatidylethanolamine, confirming that direct acylation of lysointermediates could occur in brain.
177 citations
148 citations
TL;DR: Hexaenoic phosphatidyl choline was confirmed to be a major product of methylation of phosph atidyl ethanolamine in rat liver and tetraenoic subfractions of both phospholipids showed the lowest metabolic reactivity.
113 citations
TL;DR: Selectivity of diglycerides used for the biosynthesis of either TG or phospholipids or both is substantiated by the lack of agreement between the TG carbon number distribution determined experimentally and the distribution calculated from values of either PC or PE diglyceride acetates plus the values of the 3-position of the TG.
95 citations