L
L S Nielsen
Researcher at Finsen Laboratory
Publications - 19
Citations - 2139
L S Nielsen is an academic researcher from Finsen Laboratory. The author has contributed to research in topics: Plasminogen activator & Urokinase. The author has an hindex of 14, co-authored 19 publications receiving 2126 citations. Previous affiliations of L S Nielsen include Mental Health Services.
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Journal ArticleDOI
The human receptor for urokinase plasminogen activator. NH2-terminal amino acid sequence and glycosylation variants.
Niels Behrendt,Ebbe Rønne,Michael Ploug,T Petri,Dorte Løber,L S Nielsen,W D Schleuning,Francesco Blasi,E Appella,Keld Danø +9 more
TL;DR: The receptor for human urokinase-type plasminogen activator (u-PA) was purified from phorbol 12-myristate 13-acetate-stimulated U937 cells by temperature-induced phase separation of detergent extracts, followed by affinity chromatography with immobilized diisopropyl fluorophosphate-treated u-PA.
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A 55,000-60,000 Mr receptor protein for urokinase-type plasminogen activator. Identification in human tumor cell lines and partial purification.
TL;DR: The iodinated Mr approximately equal to 15,000 amino-terminal fragment (ATF) of the urokinase-type plasminogen activator (u-PA) molecule bound specifically to the cell surface of all of seven cultured human tumor cell lines studied.
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Purification of zymogen to plasminogen activator from human glioblastoma cells by affinity chromatography with monoclonal antibody.
L S Nielsen,Jan G. Hansen,Lars Skriver,Elaine L. Wilson,Keld Kaltoft,Jesper Zeuthen,Keld Danoe +6 more
TL;DR: Findings show that the Mr 52000 human plasminogen activator exists in a proenzyme form consisting of a single polypeptide chain that by proteolysis between half-cystine residues is converted into the active enzyme consisting of two chains with molecular weights of approximately 20000 and 32000, the active site being on the latter chain.
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One-chain urokinase-type plasminogen activator from human sarcoma cells is a proenzyme with little or no intrinsic activity.
TL;DR: One-chain u-PA has a variety of properties similar to the one-chain proenzyme forms of other serine proteases and that it should, therefore, be considered as a genuine proen enzyme form of U-PA.
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Plasminogen activator inhibitor from human fibrosarcoma cells binds urokinase-type plasminogen activator, but not its proenzyme.
TL;DR: An approximately 75% pure form of a human Mr approximately 54,000 plasminogen activator inhibitor from conditioned culture fluid of the fibrosarcoma cell line HT-1080 was obtained by a single step of chromatography on concanavalin A-Sepharose.