Author
L. Schouten
Bio: L. Schouten is an academic researcher. The author has contributed to research in topics: Glyceride & Triglyceride. The author has an hindex of 2, co-authored 2 publications receiving 61 citations.
Topics: Glyceride, Triglyceride, Silica gel, Lipase, Fatty acid
Papers
More filters
TL;DR: The triglycerides of a Congo palm oil were separated into fractions by thin-layer chromatography using silica gel impregnated with silver nitrate as adsorbent to confirm the correctness of Vander Wal's theory on the distribution of fatty acids in triglyceride of natural fats.
32 citations
TL;DR: The accuracy of a method for the estimation of glycerides separated by thin-layer chromatography has been tested and found to be high as mentioned in this paper, and the results showed that the method gave equally reproducible results.
29 citations
Cited by
More filters
544 citations
TL;DR: The possibility of separating lipid materials on the basis of the number, type, and position of the unsaturated centers they contain, by virtue of the complexing of these unsaturated bonds with silver ions, provides a relatively recent but now very important addition to the range of separatory methods available to lipid chemists and biochemists.
398 citations
TL;DR: Methods for the direct quantitative analysis of spots on chromatoplates, including measurements of spot size, reflectance, absorbance of transmitted light, and fluorescence, and the photodensitometric method are described.
Abstract: Techniques for the quantitative analysis of lipids using thin-layer chromatography (TLC) are reviewed. The general procedures are divided into two groups on the basis of whether or not the methods involve the recovery of substances from chromatoplates.
167 citations
TL;DR: In this article, the effect of different variables in triglyceride separation has been studied and it was found that a longer hydrocarbon chain bonded to silica gel as well as the percent coverage improved the separation.
Abstract: High performance reversed-phase chromatography (HPRC) is an efficient and powerful tool gaining momentum in the separation of triglycerides and other lipid components. In the present study the effect of different variables in triglyceride separation has been studied. It was found that a longer hydrocarbon chain bonded to silica gel as well as the percent coverage improved the separation. Smaller particle size packing increased triglyceride resolution. A decrease in mobile phase polarity by increasing the acetone content resulted in a decrease in the retention time and resolution of triglyceride.
105 citations
TL;DR: Triglyceride lipase and diglyceride kinase can be used in a sensitive stereospecific analysis of the separate fatty acid compositions at the 1, 2 and 3 positions of a triglyceride.
Abstract: Triglyceride lipase and diglyceride kinase can be used in a sensitive stereospecific analysis of the separate fatty acid compositions at the 1, 2 and 3 positions of a triglyceride.Diglyceride kinase fromEscherichia coli selectively catalyzes the phosphorylation of 1,2-diglycerides but not the 2,3-diglycerides.The composition of the 3-position in rat liver triglycerides is clearly different from that at the 1-position.
96 citations