Author
Laëtitia Prut
Other affiliations: François Rabelais University
Bio: Laëtitia Prut is an academic researcher from French Institute of Health and Medical Research. The author has contributed to research in topics: Radial arm maze & Anxiogenic. The author has an hindex of 2, co-authored 3 publications receiving 2348 citations. Previous affiliations of Laëtitia Prut include François Rabelais University.
Papers
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TL;DR: Compounds that have a different spectrum of therapeutic efficacy in anxiety disorders such as panic attacks, generalized anxiety disorder or obsessive-compulsive disorder were poorly effective as anxiolytics in the open field test, suggesting that this paradigm may not model features of anxiety disorders.
2,665 citations
TL;DR: P/p mice are more sensitive than WT to the sensory motor, anxiolytic and amnesic effect of CDP, associated with the presence of a haplotypic block on the murine chromosome 7 and with an up regulation of gabra5 mRNAs in hippocampi of p/p F2 mice.
7 citations
Journal Article•
TL;DR: In this article, the authors propose d'etudier quelles reponses aux benzodiazepines pourraient etre expliquees par la sous-unite alpha5 du recepteur GABAA.
Abstract: Les benzodiazepines sont connues pour leurs proprietes anxiolytiques, amnesiques, hypnotiques, myorelaxantes et anticonvulsivantes. Ces dernieres pourraient etre attribuees a la sous-unite alpha du recepteur GABAA. La sous-unite alpha1 apparait comme etant responsable de l'effet sedatif et la sous-unite alpha2 de l'effet anxiolytique. Cependant, les effets attribuables aux sous-unites alpha3 et alpha5 restent a elucider. Ce travail propose d'etudier quelles reponses aux benzodiazepines pourraient etre expliquees par la sous-unite alpha5 du recepteur GABAA. Nous avons utilise des souris de generation F2 issues du croisement C57BL/6J X ABP/Le. Cette derniere lignee possede la mutation p (pink-eyed dilution, chromosome 7) localisee a 0,5 cM du locus alpha5 (gabra5) constituant un bon marqueur du gene de la sous-unite alpha5 mais egalement supposee influencer l'expression de ce dernier. Une etude comportementale de souris traitees au chlordiazepoxide fut tout d'abord entreprise et ensuite, la sous-unite alpha5 etant principalemet exprimee dans l'hippocampe, la morphologie hippocampique a ete evaluee en utilisant la coloration au nitrate d'argent (dite de Timm). L'effet amnesique et myorelaxant des benzodiazepines n'a pas ete observe chez les souris p/p. Cependant, une difference de sensibilite au pentylenetetrazole entre les souris ?/+ et p/p et entre les souris ABP/Le et C57BL/6J fut detectee. Ces resultats nous permettent de penser qu'il y aurait une implication d'un locus proche du locus p dans ces reponses aux benzodiazepines.
1 citations
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TL;DR: The tail suspension test is a useful test for assessing the behavioural effects of antidepressant compounds and other pharmacological and genetic manipulations relevant to depression.
1,326 citations
TL;DR: The present article focuses in particular upon the multifarious and complex roles of individual modulators, often as a function of the specific receptor type and neuronal substrate involved in their actions; novel targets for the management of anxiety disorders; the influence of neurotransmitters and other agents upon performance in the VCT; data acquired from complementary pharmacological and genetic strategies and, finally, several open questions likely to orientate future experimental- and clinical-research.
926 citations
TL;DR: It is shown Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.
Abstract: Animal models have proven to be invaluable to researchers trying to answer questions regarding the mechanisms of behavior. The Open Field Maze is one of the most commonly used platforms to measure behaviors in animal models. It is a fast and relatively easy test that provides a variety of behavioral information ranging from general ambulatory ability to data regarding the emotionality of the subject animal. As it relates to rodent models, the procedure allows the study of different strains of mice or rats both laboratory bred and wild-captured. The technique also readily lends itself to the investigation of different pharmacological compounds for anxiolytic or anxiogenic effects. Here, a protocol for use of the open field maze to describe mouse behaviors is detailed and a simple analysis of general locomotor ability and anxiety-related emotional behaviors between two strains of C57BL/6 mice is performed. Briefly, using the described protocol we show Wild Type mice exhibited significantly less anxiety related behaviors than did age-matched Knock Out mice while both strains exhibited similar ambulatory ability.
869 citations
TL;DR: Several novel effects of chronic, but not subchronic, antidepressant treatment are presented, including increased swimming and reduced immobility in the forced swim test and anxiety-related measures were reduced by 18 mg/kg/day.
616 citations
TL;DR: It is demonstrated that BACE1, enriched in neurons of the CNS, is a major determinant that predisposes the brain to Aβ amyloidogenesis, and future studies should be alert to potential mechanism-based side effects that may occur with BACE2 and α-secretase anti-amyloidogenic activities in neurons.
Abstract: A transmembrane aspartyl protease termed beta-site APP cleavage enzyme 1 (BACE1) that cleaves the amyloid-beta precursor protein (APP), which is abundant in neurons, is required for the generation of amyloid-beta (Abeta) peptides implicated in the pathogenesis of Alzheimer's disease (AD). We now demonstrate that BACE1, enriched in neurons of the CNS, is a major determinant that predisposes the brain to Abeta amyloidogenesis. The physiologically high levels of BACE1 activity coupled with low levels of BACE2 and alpha-secretase anti-amyloidogenic activities in neurons is a major contributor to the accumulation of Abeta in the CNS, whereas other organs are spared. Significantly, deletion of BACE1 in APPswe;PS1DeltaE9 mice prevents both Abeta deposition and age-associated cognitive abnormalities that occur in this model of Abeta amyloidosis. Moreover, Abeta deposits are sensitive to BACE1 dosage and can be efficiently cleared from the CNS when BACE1 is silenced. However, BACE1 null mice manifest alterations in hippocampal synaptic plasticity as well as in performance on tests of cognition and emotion. Importantly, memory deficits but not emotional alterations in BACE1(-/-) mice are prevented by coexpressing APPswe;PS1DeltaE9 transgenes, indicating that other potential substrates of BACE1 may affect neural circuits related to emotion. Our results establish BACE1 and APP processing pathways as critical for cognitive, emotional, and synaptic functions, and future studies should be alert to potential mechanism-based side effects that may occur with BACE1 inhibitors designed to ameliorate Abeta amyloidosis in AD.
546 citations