Lawrence A. Loeb

TL;DR: These assays illustrate mutagenic replication of oh8Gua as template causing G----T substitutions and misincorporation of Oh8G Hua as substrate causing A----C substitutions, both caused by oh8 Gua.

TL;DR: It is argued that an early step in tumor progression is one that induces a mutator phenotype, and an increased mutation rate in tumors could be the basis for the multiple mutations that characterize many cancers.

TL;DR: A broadly applicable approach to calculate x factors is developed and demonstrated using the human DNA repair enzyme 3-methyladenine DNA glycosylase (AAG) and the results are relevant to such diverse topics as applied molecular evolution, the rate of introduction of deleterious alleles into genomes in evolutionary history, and organisms' tolerance of mutational burden.

TL;DR: The high error rate of HIV-1 reverse transcriptase in vitro translates to approximately five to ten errors per HIV- 1 genome per round of replication in vivo, which suggests that misincorporation by AIDS virus is, at least in part, responsible for the hypermutability of the AIDS virus.

TL;DR: It is determined that Duplex Sequencing has a theoretical background error rate of less than one artifactual mutation per billion nucleotides sequenced and that detection of mutations present in only one of the two strands of duplex DNA can be used to identify sites of DNA damage.