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Lee Burpee

Bio: Lee Burpee is an academic researcher. The author has contributed to research in topics: Rhizoctonia & Identification (biology). The author has an hindex of 3, co-authored 3 publications receiving 1317 citations.

Papers
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15 Oct 1991
TL;DR: Identification of Rhizoctonia species, identification of rhiz octonia species and identification of Rhiza species were discussed in this paper, where the identification of the species was performed by identifying the rhiza species.
Abstract: Identification of Rhizoctonia species , Identification of Rhizoctonia species , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

596 citations

Book
01 Jan 1998
TL;DR: The phytochemical properties of Rhizoctonia mellifera have been investigated and suggest that it is a good feedstock for other phytochemicals such as phosphorous and nitrogen.
Abstract: Identification of Rhizoctonia species , Identification of Rhizoctonia species , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی

67 citations


Cited by
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Journal ArticleDOI
TL;DR: In response to threats to orchid species, integrated conservation approaches have been adopted (including ex situ and translocation principles) in the South West Australian Floristic Region with the result that a significant, multidisciplinary approach is under development to facilitate conservation of some of the most threatened taxa.

548 citations

Journal ArticleDOI
TL;DR: Comparing anastomosis reactions, rDNA-internal transcribed spacer (ITS) sequences, and virulence of isolates representing Rhizoctonia solani AG-BI and six subsets of anastsomosis group (AG)-2 found grouping based on virulence does not conform to established grouping patterns within AG-2 and does not seem useful as a group-defining criterion.
Abstract: Hyphal anastomosis reactions, rDNA-internal transcribed spacer (ITS) sequences, and virulence of isolates representing Rhizoctonia solani AG-BI and six subsets of anastomosis group (AG)-2 (-2-1, -2-2 IIIB, -2-2 IV, -2-2 LP, -2-3, and -2-4) were compared. AG-2-4 is a subset described for the first time in this report. Anastomosis reactions within AG-BI and the listed subsets of AG-2 were generally strong but, between subsets, ranged from strong to a very weak "bridging" -type reaction. Anastomosis reaction alone generally did not provide adequate evidence for placement of an isolate into a subset of AG-2. Anastomosis reactions between AG-BI and the original subsets of AG-2 (-2-1 and -2-2) are very strong; for this reason, we propose that it be included as a subset of AG-2 (designation AG-2 BI). Subsets -2-3 and -2-4 show very weak bridging-type anastomosis reactions with all other subsets of AG-2 and thus may be candidates for independent AG status. Grouping within AG-2 based on rDNA-ITS sequences was consistent with the abovementioned subsets. However, grouping based on virulence as measured herein does not conform to established grouping patterns within AG-2 and does not seem useful as a group-defining criterion. A broad range of damage was observed among members of the most virulent subsets (-2-1, -2-2 IIIB, -2-2 IV, and -2-4), whereas other subsets (-2 BI, -2-2 LP, and -2-3) were similar to one another in causing a minimal level of damage. Group-specific primer pairs for each of the seven subsets of AG-2 were designed based on the abovementioned rDNA-ITS sequences. Primer pairs proved dependable and subset specific in polymerase chain reaction amplifications of purified genomic DNA from 109 isolates of R. solani and two isolates of binucleate Rhizoctonia. These primers will provide a simple and useful method for subset-specific characterization within AG-2 if further critical evaluations confirm their specificity.

261 citations

Journal ArticleDOI
TL;DR: The internal transcribed spacer (ITS) region of nuclear ribosomal DNA from 26 isolates was sequenced and identified four fungal lineages, all related to Ceratobasidium spp.
Abstract: All orchids have an obligate relationship with mycorrhizal symbionts. Most orchid mycorrhizal fungi are classified in the form-genus Rhizoctonia. This group includes anamorphs of Tulasnella, Ceratobasidium, and Thanatephorus. Rhizoctonia can be classified according to the number of nuclei in young cells (multi-, bi-, and uninucleate). From nine Puerto Rican orchids we isolated 108 Rhizoctonia-like fungi. Our isolates were either bi- or uninucleate, the first report of uninucleate Rhizoctonia-like fungi as orchid endophytes. We sequenced the internal transcribed spacer (ITS) region of nuclear ribosomal DNA from 26 isolates and identified four fungal lineages, all related to Ceratobasidium spp. from temperate regions. Most orchid species hosted more than one lineage, demonstrating considerable variation in mycorrhizal associations even among related orchid species. The uninucleate condition was not a good phylogenetic character in mycorrhizal fungi from Puerto Rico. All four lineages were represented by fungi from Tolumnia variegata, but only one lineage included fungi from Ionopsis utricularioides. Tropical epiphytic orchids appear to vary in degree of specificity in their mycorrhizal interactions more than previously thought.

253 citations

Journal ArticleDOI
TL;DR: Results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.
Abstract: Sequence analysis of the rDNA region containing the internal transcribed spacer (ITS) regions and the 5.8s rDNA coding sequence was used to evaluate the genetic diversity of 45 isolates within and between anastomosis groups (AGs) in Rhizoctonia solani. The 5.8s rDNA sequence was completely conserved across all the AGs examined, whereas the ITS rDNA sequence was found to be highly variable among isolates. The sequence homology in the ITS regions was above 96% for isolates of the same subgroup, 66-100% for isolates of different subgroups within an AG, and 55-96% for isolates of different AGs. In neighbor-joining trees based on distances derived from ITS-5.8s rDNA sequences, subgroups IA, IB and IC within AG-1 and subgroups HG-I and HG-II within AG-4 were placed on statistically significant branches as assessed by bootstrap analysis. These results suggest that sequence analysis of ITS rDNA regions of R. solani may be a valuable tool for identifying AG subgroups of biological significance.

212 citations

Journal ArticleDOI
TL;DR: The results suggest that the B. subtilis and B. lentimorbus isolates studied have an excellent potential to be used as biocontrol agents of R. solani in tomato greenhouses at the field level.

210 citations