Liang Gao

TL;DR: Scanned Bessel beams are used in conjunction with structured illumination and/or two-photon excitation to create thinner light sheets better suited to three-dimensional (3D) subcellular imaging.

TL;DR: It is reported that rapid three-dimensional dynamics can be studied beyond the diffraction limit in thick or densely fluorescent living specimens over many time points by combining ultrathin planar illumination produced by scanned Bessel beams with super-resolution structured illumination microscopy.

TL;DR: The fundamental working principles of Bessel beam plane illumination microscopy are explained, the pros and cons of each operational mode are discussed, and examples of how to optimize experimental parameters are shown.

TL;DR: A high-spatiotemporal-resolution, multidimensional traction force microscopy system is developed to measure and model the full 3D nature of cellular forces on planar 2D surfaces and suggests that rotational moments can be generated largely via shear lag transfer to the underlying ECM from actomyosin contractility applied at the intracellular surface of a rigid adhesion of finite thickness.

TL;DR: A shoebox-sized, 10-kg, handheld mass spectrometer, Mini 10, based on a rectilinear ion trap mass analyzer has been designed, built, and characterized.