scispace - formally typeset
Search or ask a question
Author

Liangmin Huang

Bio: Liangmin Huang is an academic researcher from Chinese Academy of Sciences. The author has contributed to research in topics: Phytoplankton & Estuary. The author has an hindex of 27, co-authored 132 publications receiving 2796 citations.


Papers
More filters
Journal ArticleDOI
TL;DR: In this paper, the authors conducted a cruise along the Pearl River estuary and adjacent coastal waters south of Hong Kong during July 17 to 18, 1999, taking samples for salinity and nutrients (NO3, SiO4, PO4, NH4 and urea).
Abstract: Anthropogenic loading of nutrients in rivers often increases disproportionally among N, P, and Si, and thus may shift the type of phytoplankton nutrient limitation in the coastal receiving waters. The effect of anthropogenic nutrient loading has rarely been addressed in the Pearl River estuary along the southern coast of China, even though it is one of the largest rivers in the world. We conducted a cruise along the Pearl River estuary and adjacent coastal waters south of Hong Kong during July 17 to 18, 1999. Samples were taken for salinity and nutrients (NO3, SiO4, PO4, NH4 and urea) and nutrient addition experiments were conducted on board. Vertical profiles of salinity showed a salt-wedge estuary and the coastal plume covering the waters south of Hong Kong. Con- centrations of NO3 were very high (ca 90 µM) upstream of the Pearl River estuary, and much of the riverine NO3 was not utilized in the estuary until depletion at the edge of the coastal plume on the east side of Hong Kong. SiO4 was 120 µM upstream and its utilization was similar to that of NO3. PO4 was low in surface waters (<0.5 µM) and higher below the halocline in the estuary. NH4 and urea were generally < 4 and 1.5 µM, respectively. In the estuary, N:P ratio was 200:1, indicating potential P limitation, while N:Si was below 1:1. Beyond the coastal plume to the east of Hong Kong, N:P and N:Si ratios were <5:1 and 1:0.3, respectively, indicating potential N limitation. Nutrient limitation was shown in nutrient addition experiments and was consistent with the ratios of nutrients. There- fore, nutrient limitation shifted across the coastal plume from P limitation in the estuary to N limita- tion in the oceanic waters. Potential P limitation was observed in the estuary; P and Si co-limiting occurred at the edge of the coastal plume, and N was limiting in the oceanic side. This spatial shift in nutrient limitation has great implications for nutrient pollution control and coastal management of Hong Kong waters.

200 citations

Journal ArticleDOI
15 Dec 2016-Nature
TL;DR: The sequencing and de novo assembly of the genome of the tiger tail seahorse, Hippocampus comes is reported and it is found that the H. comes genome lacks enamel matrix protein-coding proline/glutamine-rich secretory calcium-binding phosphoprotein genes, which might have led to the loss of mineralized teeth.
Abstract: Seahorses have a specialized morphology that includes a toothless tubular mouth, a body covered with bony plates, a male brood pouch, and the absence of caudal and pelvic fins. Here we report the sequencing and de novo assembly of the genome of the tiger tail seahorse, Hippocampus comes. Comparative genomic analysis identifies higher protein and nucleotide evolutionary rates in H. comes compared with other teleost fish genomes. We identified an astacin metalloprotease gene family that has undergone expansion and is highly expressed in the male brood pouch. We also find that the H. comes genome lacks enamel matrix protein-coding proline/glutamine-rich secretory calcium-binding phosphoprotein genes, which might have led to the loss of mineralized teeth. tbx4, a regulator of hindlimb development, is also not found in H. comes genome. Knockout of tbx4 in zebrafish showed a 'pelvic fin-loss' phenotype similar to that of seahorses.

162 citations

Journal ArticleDOI
TL;DR: Results indicated 130 species of phytoplankton in the samples from the rainy season, and 132 species in the dry season, with distribution of the dominant species varied with salinity of sea-water, and their amounts correlated negatively with nutrients and zooplankon.

153 citations

Journal ArticleDOI
15 Feb 2010
TL;DR: The dynamics of size-fractionated phytoplankton along the salinity gradient in the Pearl River Estuary and the adjacent near-shore oceanic water was investigated using microscopic, flow cytometric, and chlorophyll analyses in the early spring (March) and early autumn (September) of 2005 as mentioned in this paper.
Abstract: The dynamics of size-fractionated phytoplankton along the salinity gradient in the Pearl River Estuary and the adjacent near-shore oceanic water was investigated using microscopic, flow cytometric, and chlorophyll analyses in the early spring (March) and early autumn (September) of 2005. In the inner part of the estuary where salinity was less than 30, the phytoplankton community was dominated by micro- and nano-sized (3-200 mu m) cells, particularly the diatom Skeletonema costatum, both in early spring and early autumn. In areas where salinity > 30, including the mixing zone and nearshore oceanic water, micro- and nano-sized cell populations dominated the phytoplankton assemblage during early spring when influence of river discharge was minimal, whereas pico-sized ( 30) areas as a result of short residence time, exerting a strong influence on phytoplankton abundance, especially picophytoplankton in the nearshore, otherwise oligotrophic, water. Influenced by high abundance of DIN and limitation in phosphorus, picophytoplankton in the adjacent nearshore oceanic water rose to prominence seasonally. Our results indicate that eutrophication in the Pearl River Estuary not only stimulates the growth of S. costatum in the nutrient-rich areas of the estuary but also appears to promote the growth of Synechococcus and pico-eukaryotes in the adjacent usually oligotrophic oceanic water at least during our autumn cruise. (C) 2009 Elsevier Ltd. All rights reserved.

113 citations

Journal ArticleDOI
01 Oct 2004
TL;DR: In this article, the authors investigated the spatial distribution of primary productivity, and two size fractionations (5 pm) of chlorophyll in the Pearl River estuary and across the coastal plume south of Hong Kong during the season of high river discharge (July, 1999 and 2000).
Abstract: The Pearl River estuary fuels phytoplankton productivity in the estuarine coastal waters in the oligotrophic South China Sea. Cruises were conducted to investigate the spatial distribution of primary productivity, and two size fractionations ( 5 pm) of chlorophyll in the Pearl River estuary and across the coastal plume south of Hong Kong during the season of high river discharge (July, 1999 and 2000). During 18-19 July 1999, phytoplankton biomass and productivity were low in the freshwater dominated estuary ( 5 mum size fractionation (> 50%). The Pearl River estuary displayed a typical spatial variability of phytoplankton biomass: low biomass and productivity due to rapid dilution and light limitation in turbid estuarine waters, a regional maximum of biomass and productivity at intermediate salinities in the coastal plume and low biomass due to nutrient limitation in oceanic waters. In summer when south-southwest monsoonal winds blow, part of the coastal plume moves eastwards. A regional maximum of phytoplankton usually develops in the coastal plume south of Hong Kong. However, a change in wind direction to the east or northeast can cause the following effects: pushing the coastal plume westward to the estuary, holding (retaining) the estuarine plume in the estuary (due to the Coriolis effect) and at the same time, mixing the water column vertically. Such a strong east or northeast wind event was observed during July 22-26 1999 and caused an increase in salinity at the surface in the estuary compared to before the wind event. This wind event increased the residence time of the estuarine plume inside the estuary, and as a result, a phytoplankton bloom (52-151 mg chl am(-2)) developed during July 25-26 inside the estuary. This similar easterly wind effect was observed during July 2000. After a strong easterly wind event (speed 10 ms(-1)) on July 11, the coastal plume was absent in waters south of Hong Kong on July 13, but it returned on July 19 after winds blew from the south and southwest direction. The results suggest that wind events not only changed the spatial movement of the coastal plume, but also the spatial distribution of phytoplankton biomass and productivity in the Pearl River estuary and adjacent coastal waters. (C) 2004 Elsevier Ltd. All rights reserved.

109 citations


Cited by
More filters
Journal Article
Fumio Tajima1
30 Oct 1989-Genomics
TL;DR: It is suggested that the natural selection against large insertion/deletion is so weak that a large amount of variation is maintained in a population.

11,521 citations

Book ChapterDOI
01 Jan 1976
TL;DR: A positive temperature coefficient is the term which has been used to indicate that an increase in solubility occurs as the temperature is raised, whereas a negative coefficient indicates a decrease in Solubility with rise in temperature.
Abstract: A positive temperature coefficient is the term which has been used to indicate that an increase in solubility occurs as the temperature is raised, whereas a negative coefficient indicates a decrease in solubility with rise in temperature.

1,573 citations

Journal Article
TL;DR: The comparison of related genomes has emerged as a powerful lens for genome interpretation as mentioned in this paper, which reveals a small number of new coding exons, candidate stop codon readthrough events and over 10,000 regions of overlapping synonymous constraint within protein-coding exons.
Abstract: The comparison of related genomes has emerged as a powerful lens for genome interpretation. Here we report the sequencing and comparative analysis of 29 eutherian genomes. We confirm that at least 5.5% of the human genome has undergone purifying selection, and locate constrained elements covering ∼4.2% of the genome. We use evolutionary signatures and comparisons with experimental data sets to suggest candidate functions for ∼60% of constrained bases. These elements reveal a small number of new coding exons, candidate stop codon readthrough events and over 10,000 regions of overlapping synonymous constraint within protein-coding exons. We find 220 candidate RNA structural families, and nearly a million elements overlapping potential promoter, enhancer and insulator regions. We report specific amino acid residues that have undergone positive selection, 280,000 non-coding elements exapted from mobile elements and more than 1,000 primate- and human-accelerated elements. Overlap with disease-associated variants indicates that our findings will be relevant for studies of human biology, health and disease.

926 citations

Journal ArticleDOI
TL;DR: The first spatially explicit, multielement (N, P, and C), multiform (dissolved inorganic: DIN, DIP; dissolved organic: DOC, DON, DOP; and particulate: POC, PN, PP) predictive model system of river nutrient export from watersheds (Global Nutrient export from Watersheds (NEWS)) is presented in this paper.
Abstract: [1] An overview of the first spatially explicit, multielement (N, P, and C), multiform (dissolved inorganic: DIN, DIP; dissolved organic: DOC, DON, DOP; and particulate: POC, PN, PP) predictive model system of river nutrient export from watersheds (Global Nutrient Export from Watersheds (NEWS)) is presented. NEWS models estimate export from 5761 watersheds globally as a function of land use, nutrient inputs, hydrology, and other factors; regional and global scale patterns as of 1995 are presented here. Watershed sources and their relative magnitudes differ by element and form. For example, anthropogenic sources dominate the export of DIN and DIP at the global scale, although their anthropogenic sources differ significantly (diffuse and point, respectively). Natural sources dominate DON and DOP export globally, although diffuse anthropogenic sources dominate in several regions in Asia, Europe and N. America. “Hot spots” where yield (kg km−2 yr−1) is high for several elements and forms were identified, including parts of Indonesia, Japan, southern Asia, and Central America, due to anthropogenic N and P inputs in some regions and high water runoff in others. NEWS models provide a tool to examine past, current and future river export of nutrients, and how humans might impact element ratios and forms, and thereby affect estuaries and coastal seas.

641 citations

Journal Article

626 citations