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Ling-Ling Yang

Bio: Ling-Ling Yang is an academic researcher from Dalian Institute of Chemical Physics. The author has contributed to research in topics: Illumina dye sequencing & RNA-Seq. The author has an hindex of 1, co-authored 1 publications receiving 178 citations.

Papers
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Journal ArticleDOI
22 Jun 2011-PLOS ONE
TL;DR: The de novo assembly of Taxus mairei transcriptome using Illumina paired-end sequencing technology provides the most comprehensive sequence resource available for Taxus study and will help define mechanisms of tissue specific functions and secondary metabolism in non-model plant organisms.
Abstract: Background Illumina second generation sequencing is now an efficient route for generating enormous sequence collections that represent expressed genes and quantitate expression level. Taxus is a world-wide endangered gymnosperm genus and forms an important anti-cancer medicinal resource, but the large and complex genomes of Taxus have hindered the development of genomic resources. The research of its tissue-specific transcriptome is absent. There is also no study concerning the association between the plant transcriptome and metabolome with respect to the plant tissue type.

188 citations

Journal ArticleDOI
TL;DR: In this article , a pattern-reconfigurable dielectric resonator antenna (DRA) with compact structure and high efficiency is proposed by introducing a pair of switchable directors.
Abstract: A novel pattern-reconfigurable dielectric resonator antenna (DRA) with compact structure and high efficiency is proposed in this letter by introducing a pair of switchable directors. The switchable directors are arranged on both sides of an omnidirectional DRA, which operates in the TE01δ mode. Their directing function can be controlled by switching the on/off status of the p-i-n diodes so that one omnidirectional radiation pattern and two unidirectional endfire patterns are provided in the azimuthal plane. To verify this idea, a prototype of the proposed antenna is fabricated and measured. Good agreement between the simulated and measured results can be observed. The peak gain in endfire status reaches 4.5 dBi and the front-to-back ratio is more than 12 dB. Meanwhile, the gain variation of the omnidirectional pattern in the azimuth plane is less than 2 dB, which shows good omnidirectional radiation performance.

12 citations

DOI
TL;DR: In this paper , a novel pattern-reconfigurable dielectric resonator (DR) antenna with end-fire beam-scanning feature is proposed, which consists of a DR, a switchable director with 10 p-i-n diodes, and a differential feeding structure.
Abstract: A novel pattern-reconfigurable dielectric resonator (DR) antenna with endfire beam-scanning feature is proposed in this letter. The proposed antenna consists of a DR, a switchable director with 10 p-i-n diodes, and a differential feeding structure. The differential-fed DR operating in TE01δ mode can provide a primary endfire pattern with wide beam. Based on this, a large-coverage beam-scanning performance can be obtained by introducing a switchable director placed in front of the DR. A prototype is fabricated and measured to verify the proposed design. By switching the p-i-n diodes of the switchable director, the proposed antenna enables a near-continuous beam scanning from −48° to +48° in the azimuth plane with a gain fluctuation of less than 0.5 dB. Besides, the 3 dB scanning coverage is up to 164° (from −82° to +82°), which shows good beam-scanning performance.

4 citations

DOI
TL;DR: In this article, a simple and effective self-decoupling method for dielectric patch (DP) antenna (DPA) by choosing proper structural parameters, without adding extra components, is presented.
Abstract: This letter presents a simple and effective self-decoupling method for dielectric patch (DP) antenna (DPA) by choosing proper structural parameters, without adding extra components. By theoretical analysis, the attenuation constant of the employed fundamental TM01 mode outside the DP is controlled by the structural parameters of the DP resonator, which is crucial for the decoupling between the two adjacent DPAs. It is found that the larger the attenuation constant of the TM01 mode, the better the port-to-port isolation in broadband, and then the radiation pattern can be stabilized. Under the premise of satisfactory isolation, the operational bandwidth and boresight gain of the DPA are studied for ensuring good antenna performance, exhibiting high design freedom and flexibility of the DPA. For demonstration, 1 × 2 closely spaced DPAs with optimal parameters are fabricated and measured. The simulated and measured results show good agreement.

2 citations

DOI
10 Jul 2022
TL;DR: In this paper , a wideband decoupling method for dielectric patch (DP) antenna (DPA) is proposed, which can not only incorporate TM10 and antiphase TM20 modes to expand the antenna bandwidth, but also make the port-to-port isolation (S21) flat in broadband.
Abstract: A wideband decoupling method for dielectric patch (DP) antenna (DPA) is proposed in this paper. The air tunnel can not only incorporate TM10 and antiphase TM20 modes to expand the antenna bandwidth, but also make the port-to-port isolation (S21) flat in broadband. Furthermore, the two pairs of metal strips on the DPA help to achieve a balance between the mutual capacitive and inductive coupling, further improving wideband isolation. Meanwhile, it is worth noting that the radiation pattern can be stabilized during the decoupling process. Finally, the simulation result exhibits that the impedance bandwidth is 18.4% (4.97GHz-5.98GHz) and the in-band isolation is enhanced to more than 20dB.

1 citations


Cited by
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Journal ArticleDOI
TL;DR: The Aedes albopictus Foshan strain has a large genome with significant population-based size variation, the largest mosquito genome sequenced to date, and its size results principally from an abundance of repetitive DNA classes.
Abstract: The Asian tiger mosquito, Aedes albopictus, is a highly successful invasive species that transmits a number of human viral diseases, including dengue and Chikungunya fevers. This species has a large genome with significant population-based size variation. The complete genome sequence was determined for the Foshan strain, an established laboratory colony derived from wild mosquitoes from southeastern China, a region within the historical range of the origin of the species. The genome comprises 1,967 Mb, the largest mosquito genome sequenced to date, and its size results principally from an abundance of repetitive DNA classes. In addition, expansions of the numbers of members in gene families involved in insecticide-resistance mechanisms, diapause, sex determination, immunity, and olfaction also contribute to the larger size. Portions of integrated flavivirus-like genomes support a shared evolutionary history of association of these viruses with their vector. The large genome repertory may contribute to the adaptability and success of Ae. albopictus as an invasive species.

248 citations

Journal ArticleDOI
27 Apr 2012-PLOS ONE
TL;DR: Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots, tissue-specific gene expression, potential biotic and abiotic stress response in sweet potato.
Abstract: Background Sweet potato (Ipomoea batatas L. [Lam.]) ranks among the top six most important food crops in the world. It is widely grown throughout the world with high and stable yield, strong adaptability, rich nutrient content, and multiple uses. However, little is known about the molecular biology of this important non-model organism due to lack of genomic resources. Hence, studies based on high-throughput sequencing technologies are needed to get a comprehensive and integrated genomic resource and better understanding of gene expression patterns in different tissues and at various developmental stages. Methodology/Principal Findings Illumina paired-end (PE) RNA-Sequencing was performed, and generated 48.7 million of 75 bp PE reads. These reads were de novo assembled into 128,052 transcripts (≥100 bp), which correspond to 41.1 million base pairs, by using a combined assembly strategy. Transcripts were annotated by Blast2GO and 51,763 transcripts got BLASTX hits, in which 39,677 transcripts have GO terms and 14,117 have ECs that are associated with 147 KEGG pathways. Furthermore, transcriptome differences of seven tissues were analyzed by using Illumina digital gene expression (DGE) tag profiling and numerous differentially and specifically expressed transcripts were identified. Moreover, the expression characteristics of genes involved in viral genomes, starch metabolism and potential stress tolerance and insect resistance were also identified. Conclusions/Significance The combined de novo transcriptome assembly strategy can be applied to other organisms whose reference genomes are not available. The data provided here represent the most comprehensive and integrated genomic resources for cloning and identifying genes of interest in sweet potato. Characterization of sweet potato transcriptome provides an effective tool for better understanding the molecular mechanisms of cellular processes including development of leaves and storage roots, tissue-specific gene expression, potential biotic and abiotic stress response in sweet potato.

153 citations

Journal ArticleDOI
TL;DR: The present study identified potential genes involved in salt tolerance of R. trigyna and identified a useful source of reference sequences for closely related taxa, which can further the understanding of salt tolerance in other halophytes surviving under sodic stress.
Abstract: Reaumuria trigyna is an endangered small shrub endemic to desert regions in Inner Mongolia. This dicotyledonous recretohalophyte has unique morphological characteristics that allow it to tolerate the stress imposed by semi-desert saline soil. However, it is impossible to explore the mechanisms underlying this tolerance without detailed genomic information. Fortunately, newly developed high-throughput sequencing technologies are powerful tools for de novo sequencing to gain such information for this species. Two sequencing libraries prepared from control (C21) and NaCl-treated samples (T43) were sequenced using short reads sequencing technology (Illumina) to investigate changes in the R. trigyna transcriptome in response to salt stress. Among 65340 unigenes, 35495 (52.27%) were annotated with gene descriptions, conserved domains, gene ontology terms, and metabolic pathways with a cut-off E-value of 10-5. These included 44 Gene Ontology (GO) terms, 119 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, and 25 Clusters of Orthologous Groups families. By comparing the transcriptomes from control and NaCl-treated plants, 5032 genes showed significantly differences in transcript abundance under salt stress (false discovery rate ≤ 0.001 and |log2Ratio| ≥ 1). These genes were significantly enriched in 29 KEGG pathways and 26 GO terms. The transcription profiles indicated that genes related to ion transport and the reactive oxygen species scavenging system were relevant to the morphological and physiological characteristics of this species. The expression patterns of 30 randomly selected genes resulted from quantitative real-time PCR were basically consistent with their transcript abundance changes identified by RNA-seq. The present study identified potential genes involved in salt tolerance of R. trigyna. The globally sequenced genes covered a considerable proportion of the R. trigyna transcriptome. These data represent a genetic resource for the discovery of genes related to salt tolerance in this species, and may be a useful source of reference sequences for closely related taxa. These results can also further our understanding of salt tolerance in other halophytes surviving under sodic stress.

149 citations

Journal ArticleDOI
11 Oct 2012-PLOS ONE
TL;DR: This is the first study that used a next-generation high-throughput DNA sequencing technique, the Solexa/Illumina GA II method, to analyze the transcriptome from whole bodies of L. vannamei larvae, which will facilitate the understanding of the genome background of crustaceans.
Abstract: Background Pacific white shrimp (Litopenaeus vannamei), the major species of farmed shrimps in the world, has been attracting extensive studies, which require more and more genome background knowledge. The now available transcriptome data of L. vannamei are insufficient for research requirements, and have not been adequately assembled and annotated.

147 citations

Journal ArticleDOI
TL;DR: The field of genomics is driven by technological improvements in sequencing platforms; however, software and algorithm development has lagged behind reductions in sequencing costs, improved throughput, and quality improvements as discussed by the authors.
Abstract: The study of plant biology in the 21st century is, and will continue to be, vastly different from that in the 20th century. One driver for this has been the use of genomics methods to reveal the genetic blueprints for not one but dozens of plant species, as well as resolving genome differences in thousands of individuals at the population level. Genomics technology has advanced substantially since publication of the first plant genome sequence, that of Arabidopsis thaliana, in 2000. Plant genomics researchers have readily embraced new algorithms, technologies and approaches to generate genome, transcriptome and epigenome datasets for model and crop species that have permitted deep inferences into plant biology. Challenges in sequencing any genome include ploidy, heterozygosity and paralogy, all which are amplified in plant genomes compared to animal genomes due to the large genome sizes, high repetitive sequence content, and rampant whole- or segmental genome duplication. The ability to generate de novo transcriptome assemblies provides an alternative approach to bypass these complex genomes and access the gene space of these recalcitrant species. The field of genomics is driven by technological improvements in sequencing platforms; however, software and algorithm development has lagged behind reductions in sequencing costs, improved throughput, and quality improvements. It is anticipated that sequencing platforms will continue to improve the length and quality of output, and that the complementary algorithms and bioinformatic software needed to handle large, repetitive genomes will improve. The future is bright for an exponential improvement in our understanding of plant biology.

144 citations