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Ling-Yien Hii

Bio: Ling-Yien Hii is an academic researcher from Boston Children's Hospital. The author has contributed to research in topics: Amniotic stem cells & Fallopian tube. The author has an hindex of 1, co-authored 4 publications receiving 6 citations.

Papers
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Journal ArticleDOI
TL;DR: Combination of endoscopic fetal surgery with TRASCET or tissue engineering will be a new vision to achieve to improve the outcome of prenatal intervention in fMMC.
Abstract: Purpose of review To review the advance of maternal--fetal surgery, the research of stem cell transplantation and tissue engineering in prenatal management of fetal meningomyelocele (fMMC). Recent findings Advance in the imaging study provides more accurate assessment of fMMC in utero. Prenatal maternal--fetal surgery in fMMC demonstrates favourable postnatal outcome. Minimally invasive fetal surgery minimizes uterine wall disruption. Endoscopic fetal surgery is performed via laparotomy-assisted or entirely percutaneous approach. The postnatal outcome for open and endoscopic fetal surgery shares no difference. Single layer closure during repair of fMMC is preferred to reduce postnatal surgical intervention. All maternal--fetal surgeries impose anesthetic and obstetric risk to pregnant woman. Ruptured of membrane and preterm delivery are common complications. Trans-amniotic stem cell therapy (TRASCET) showed potential tissue regeneration in animal models. Fetal tissue engineering with growth factors and dura substitutes with biosynthetic materials promote spinal cord regeneration. This will overcome the challenge of closure in large fMMC. Planning of the maternal--fetal surgery should adhere to ethical framework to minimize morbidity to both fetus and mother. Summary Combination of endoscopic fetal surgery with TRASCET or tissue engineering will be a new vision to achieve to improve the outcome of prenatal intervention in fMMC.

8 citations

Journal ArticleDOI
TL;DR: Using conditioned-Dulbecco's pluripotent stem cells from amniotic membrane stem cells (AMSCs) has the therapeutic potential to treat bovine mastitis and may replace antibiotics therapy in the future.
Abstract: Objective Bovine mastitis results in economic loss due to decrease in milk production. Antibiotic ointments are commonly used for treating. However, residue and anti-microbial resistance warranted attention progressively. Fortunately, stem cell anti-inflammatory properties and paracrine expression of cytokines accelerates wound healing and suppresses inflammatory reactions in mastitis. The objective of this study is to use the conditioned-Dulbecco's pluripotent stem cells (DPBS) from amniotic membrane stem cells (AMSCs) in treating bovine mastitis. Materials and methods The cows with mastitis were divided into two groups. In antibiotic control group, the cows were given tetraneomycin ointment. In conditioned-DPBS of AMSCs treatment group, amniotic membrane was collected for AMSCs after delivery. With expression of surface antigen and potential of tri-linage differentiation, AMSCs were injected into mammary glands. Then, milk was sampled every three days to monitor the effect of both treatments. The quality of milk was measured with pH, titratable acidity, free calcium ions and somatic cell count. Results Our results demonstrated the Bovine AMSCs expressed CD44, low levels of CD4 and no CD105. Bovine AMSCs demonstrated the differentiation capability in the tri-cell lineages. Mastitis treatment with conditioned-DPBS from AMSCs (experimental group) and conventional antibiotics (control group) showed insignificant difference in pH value and titratable acidity. The level of ionic calcium concentration in the conditioned-DPBS group decreased from 3rd day to 12th day, while the level in the antibiotic group decreased from 0 day to 12th day. The somatic cell number was similar in both groups, which meet the standard of Taiwan milk collection. Conclusion In conclusion, conditioned-DPBS from bovine AMSCs has the therapeutic potential to treat bovine mastitis and may replace antibiotics therapy in the future.

5 citations

Journal ArticleDOI
TL;DR: In this article, a 38-year-old multipara woman showed a normal karyotype and bilateral femur and humerus length symmetrically shortened after 20 weeks due to a de novo mutation in COL2A1, causing Stickler syndrome type 1.
Abstract: Objective Skeletal dysplasias, caused by genetic mutations, are a heterogenous group of heritable disorders affecting bone development during fetal life. Stickler syndrome, one of the skeletal dysplasias, is an autosomal dominant connective tissue disorder caused by abnormal collagen synthesis owing to a genetic mutation in COL2A1. Case report We present the case of a 38-year-old multipara woman whose first trimester screening showed a normal karyotype. However, the bilateral femur and humerus length symmetrically shortened after 20 weeks. Next-generation sequencing for mutations in potential genes leading to skeletal dysplasia detected a novel de novo mutation (c.1438G > A, p.Gly480Arg) in COL2A1, causing Stickler syndrome type 1. This pathogenic mutation might impair or destabilize the collagen structure, leading to collagen type II, IX, and XI dysfunction. Conclusion We identified a novel de novo mutation in COL2A1 related to the STL1 syndrome and delineated the extent of the skeletal dysplasia disease spectrum.

3 citations

Journal ArticleDOI
TL;DR: Gynecologist should increase the awareness of heterotopic pregnancy in patients receiving ART and reproductive endocrinologist should reduce the risk of high-order pregnancy without compromising pregnancy rate.
Abstract: Objective We report a rare case of heterotopic pregnancy and high-order pregnancy occurring simultaneously following the use of the assisted reproductive technique (ART). Case report A 29-year-old woman, Gravida 2 Para 1, became pregnant after receiving intrauterine insemination (IUI). She came to our emergency room due to diffuse low abdominal pain at seven weeks of gestational age. Transabdominal sonography (TAS) revealed a quadruplet intrauterine pregnancy with an enlarged left adnexa and intrapelvic fluid accumulation. Simultaneous occurrence of high-order pregnancy and left tubal pregnancy with internal hemorrhage was suspected. The patient received an emergent laparoscopic resection of the affected Fallopian tube and recovered well for the remaining hospitalization course. Afterwards, she received fetal reduction procedure and eventually gave birth to twin babies. Conclusion Gynecologist should increase the awareness of heterotopic pregnancy in patients receiving ART. On the other hand, reproductive endocrinologist should reduce the risk of high-order pregnancy without compromising pregnancy rate.

1 citations


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TL;DR: Compared with delayed clamping, cord milking yielded similar effects on hematocrit concentrations, rates of necrotizing enterocolits, and intraventricular hemorrhage, and need for neonatal transfusions, and did not carry any increased risk of complications.
Abstract: B oth delayed clamping and milking of the umbilical cord have separately been shown to have positive effects for neonates. Delayed clamping improves neonatal anemia, lowers the need for transfusion, lowers the incidence of necrotizing enterocolitis, and decreases the incidence of intraventricular hemorrhage. While milking has also been associated with improved neonatal outcomes, there is a lack of studies comparing the 2 techniques. This study aimed to compare delayed clamping and umbilical cord milking in terms of effect on the initial hematocrit concentrations in preterm infants. This randomized controlled trial included singleton infants between 23 weeks 0 days gestation and 34 weeks 6 days gestation that were born at a single hospital between April 18, 2014, and June 5, 2018. Patients were excluded with known congenital anomalies, precipitous delivery, placental abruption, uterine rupture, or infants with risk factors for anemia. Patients were randomized to either the delayed cord clamping group or milking group using 1:1 block randomization. Delayed cord clamping lasted for 60 seconds and infants were held at the level of the maternal abdomen during cesarean delivery and at the perineum for vaginal delivery. For the milking group, the cord was milked 4 times before clamping, from ∼20 cm from the placental end of the cord to the infant’s umbilicus, with time for cord refill in between milking. An intent-to-treat approach was used in analysis. The sample size was calculated to create an 80% probability value of .05 for the primary outcome measure of a hematocrit difference of 3%. Of 282 patients who were randomized, 204 were included in the analysis. Of those, 100 were assigned to the milking group and 104 were assigned to the delayed clamping group. The milking procedure took, on average, 6 seconds. The cord clamping group had an average delayed cord clamping of 30 to 60 seconds. The clamping and milking groups were similar in terms of delivery mode, birth weight, cord blood arterial, and venous pH and Apgar scores. The median gestational age for delivery was 32 weeks. While there was a trend toward higher initial hematocrit concentration in the milking group compared with the delayed clamping group, this difference was not statistically significant (51.8 vs. 49.9; P=0.07). The 2 groups were similar in terms of transfusion (15.5% in the delayed clamping group vs. 9.1% in the milking group; P=0.24), intraventricular hemorrhage (delayed clamping group, 15.5% vs. milking group, 10.1%; P=0.35), and necrotizing enterocolitis (delayed clamping group, 5.8% vs. milking group, 3.0%; P=0.49). While the differences did not reach statistical significance, all these outcomes were lower in the milking group. Compared with delayed clamping, cord milking yielded similar effects on hematocrit concentrations, rates of necrotizing enterocolits, and intraventricular hemorrhage, and need for neonatal transfusions, and did not carry any increased risk of complications. There may be certain clinical situations where a 30to 60-second delay in cord clamping may delay necessary resuscitation by the neonatal team and milking the umbilical cord may be an acceptable alternative. These outcomes support the use of milking as a method for increasing infant blood volume. Subsequent Pregnancy Outcomes After Open Maternal-Fetal Surgery for Myelomeningocele

25 citations

Journal ArticleDOI
TL;DR: The biocompatibility of fibrin hydrogel patches on the fetal spinal cord is demonstrated and this organotypic slice culture system is suggested as a useful platform for evaluating mechanisms of damage and repair in children with neural tube defects.
Abstract: Studying how the fetal spinal cord regenerates in an ex vivo model of spina bifida repair may provide insights into the development of new tissue engineering treatment strategies to better optimize...

7 citations

Journal ArticleDOI
TL;DR: In this paper , Salvia miltiorrhiza polysaccharides (SMPs) were used to prevent S. aureus-induced mastitis in dairy cows.
Abstract: Abstract The lactation capacity of dairy cows is critical to the productivity of the animals. Mastitis is a disease that directly affects the lactation capacity of cows. Staphylococcus aureus ( S. aureus ) is one of the most important pathogens that causes mastitis in dairy cows. The anti-inflammatory effect of Salvia miltiorrhiza polysaccharides (SMPs) has been demonstrated in mice and chickens. However, the effectiveness of SMPs in preventing and treating mastitis is unclear. Therefore, the purpose of this study was to explore the protective effect and mechanism of SMPs on mastitis caused by S. aureus . S. aureus was used to induce mastitis in rats, and three doses of SMPs (87.5, 175, 350 mg/kg, BW/d) were administered as treatments. The bacterial load, histopathology, and myeloperoxidase (MPO) and N-acetyl-β-D-glucosaminidase (NAGase) activities of mammary glands were observed and measured. Cytokines, including interleukin (IL)-1β, interleukin (IL)-6, and tumor necrosis factor α (TNF-α), were examined by qRT–PCR and ELISA. Key proteins in the NF-κB and MAPK signaling pathways were analyzed by Western blotting. The results showed that SMP supplementation could significantly reduce the colonization of S. aureus and the recruitment of inflammatory cells in mammary glands. S. aureus -induced gene transcription and protein expression of IL-1β, IL-6, and TNF-α were significantly suppressed in mammary glands. In addition, the increase in NF-κB and MAPK protein phosphorylation was inhibited by SMPs. These results revealed that supplementation with SMPs protected the mammary gland of rats against damage caused by S. aureus and alleviated the inflammatory response. This study provides a certain experimental basis for the treatment of S. aureus- induced mastitis with SMPs in the future.

5 citations

Journal ArticleDOI
04 Sep 2021-Bone
TL;DR: In this paper, a de novo mutation in human COL2A1 related to lethal skeletal dysplasia and expanded the mutation spectrum of type II collagenopathies was identified using bioinformatics, zebrafish models, and assisted reproduction technology (ART) combined with preimplantation genetic testing for monogenic diseases.

5 citations

24 Oct 2015
TL;DR: Both pMSC and afMSC can induce comparable rates of coverage of experimental spina bifida after concentrated intraamniotic injection in the rodent model, which broadens the options for timing and cell source for TRASCET as a potential alternative in the prenatal management of spina Bifida.
Abstract: PURPOSE We compared placental-derived and amniotic fluid-derived mesenchymal stem cells (pMSCs and afMSCs, respectively) in transamniotic stem cell therapy (TRASCET) for experimental spina bifida. METHODS Pregnant dams (n=29) exposed to retinoic acid for the induction of fetal spina bifida were divided into four groups. Three groups received volume-matched intraamniotic injections of either saline (n=38 fetuses) or a suspension of 2×10(6) cells/mL of syngeneic, labeled afMSCs (n=73) or pMSCs (n=115) on gestational day 17 (term=21-22days). Untreated fetuses served as controls. Animals were killed before term. Statistical comparisons were by Fisher's exact test (p<0.05). RESULTS Survival was similar across treatment groups (p=0.08). In fetuses with isolated spina bifida (n=100), there were higher percentages of defect coverage (either partial or complete) in both afMSC and pMSC groups compared with saline and untreated groups (p<0.001-0.03 in pairwise comparisons). There were no differences in coverage rates between afMSC and pMSC groups (p=0.94) or between saline and untreated groups (p=0.98). CONCLUSIONS Both pMSC and afMSC can induce comparable rates of coverage of experimental spina bifida after concentrated intraamniotic injection in the rodent model. This broadens the options for timing and cell source for TRASCET as a potential alternative in the prenatal management of spina bifida.

4 citations