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Luciana Relly Bertolini

Bio: Luciana Relly Bertolini is an academic researcher from Pontifícia Universidade Católica do Rio Grande do Sul. The author has contributed to research in topics: Oocyte & Somatic cell nuclear transfer. The author has an hindex of 13, co-authored 49 publications receiving 760 citations. Previous affiliations of Luciana Relly Bertolini include University of Rio Grande & University of Fortaleza.

Papers
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Journal ArticleDOI
TL;DR: The in vitro production of bovine embryos negatively affected the amount of gene expression on day 7 and the rate of development on day 16, which appeared to be significant for growth and development.
Abstract: The effects of the embryo production system on growth and transcription rate of day 7 and 16 bovine embryos were investigated. In vivo- (controls) and in vitro-produced (IVP) embryos were transferred to female recipients on day 7 of development, and were allowed to develop in a synchronous uterine environment to day 16. Embryonic transcripts for insulin-like growth factors-1 and -2 (IGF-1 and -2), their receptors (IGF-1r and -2r), facilitative glucose transporters-1 and -3 (Glut-1 and -3), and interferon-τ (IFN-τ) were determined by real-time quantitative PCR (TaqMan®); gender diagnosis was performed on day 16 concepti only. On day 7, IVP embryos presented lower mRNA levels than controls (P < 0.05), but these differences were generally reduced on day 16. No IGF-1 transcripts were detected on day 7, but a low IGF-1 mRNA level was observed in day 16 embryos. In the IVP group, IFN-τ mRNA levels were lower on day 7 (P < 0.05), but higher than controls on day 16 (P < 0.05). Control embryos showed a temporal decrease in the relative transcription from day 7 to 16 (P < 0.05), except IGF-1 mRNA. On day 16, IVP concepti were shorter and displayed smaller embryonic discs (P < 0.05). Female concepti were generally smaller than males, and IGF-2r mRNA and growth were negatively correlated. The in vitro production of bovine embryos negatively affected the amount of gene expression on day 7 and the rate of development on day 16. Physical traits and transcriptional activity on day 16 were associated with one another, which appeared to be significant for growth and development. Mol. Reprod. Dev. 63: 318–328, 2002. © 2002 Wiley-Liss, Inc.

159 citations

Journal ArticleDOI
TL;DR: In vivo pluripotency of the cells was demonstrated by birth of a chimeric piglet, documented by pigmentation and DNA markers, and the ability to direct the development of nuclear-transfer embryos to the blastocyst stage was demonstrated.

148 citations

Journal ArticleDOI
TL;DR: The approval of two mammary gland-derived recombinant proteins for commercial and clinical use has boosted the interest for more efficient, safer and economic ways to generate transgenic founders to meet the increasing demand for biomedical proteins worldwide.
Abstract: The recombinant production of therapeutic proteins for human diseases is currently the largest source of innovation in the pharmaceutical industry. The market growth has been the driving force on efforts for the development of new therapeutic proteins, in which transgenesis emerges as key component. The use of the transgenic animal platform offers attractive possibilities, residing on the low production costs allied to high productivity and quality of the recombinant proteins. Although many strategies have evolved over the past decades for the generation of transgenic founders, transgenesis in livestock animals generally faces some challenges, mainly due to random transgene integration and control over transgene copy number. But new developments in gene editing with CRISPR/Cas system promises to revolutionize the field for its simplicity and high efficiency. In addition, for the final approval of any given recombinant protein for animal or human use, the production and characterization of bioreactor founders and expression patterns and functionality of the proteins are technical part of the process, which also requires regulatory and administrative decisions, with a large emphasis on biosafety. The approval of two mammary gland-derived recombinant proteins for commercial and clinical use has boosted the interest for more efficient, safer and economic ways to generate transgenic founders to meet the increasing demand for biomedical proteins worldwide.

73 citations

Journal ArticleDOI
TL;DR: The results indicated larger concepti and increased placental fructogenic capacity in mid- to late IVP pregnancies, features which appeared to be associated with an enhanced substrate supply, potentially glucose, to the conceptus.
Abstract: Changes in placental development have been associated with foetal abnormalities after in vitro embryo manipulations. This study was designed to investigate bovine conceptus development and substrate levels in plasma and fluids in in vivo- and in vitro-produced (IVP) concepti and neonates. In vivo-produced and IVP embryos were derived by established embryo production procedures. Pregnant animals from both groups were slaughtered on days 90 or 180 of gestation, or allowed to go to term. Conceptus and neonatal physical traits were recorded; foetal, maternal and neonatal blood, and foetal fluids were collected for the determination of blood and fluid chemistry, and glucose, fructose and lactate concentrations. Placental transcripts for specific glucose transporters were determined by quantitative RT-PCR. No significant differences in uterine and conceptus traits were observed between groups on day 90. On day 180, larger uterine, placental and foetal weights, and an increase in placental gross surface area (SA) in IVP pregnancies were associated with increased glucose and fructose accumulation in foetal plasma and associated fluids, with no differences in the expression of components of the glucose transporter system. Therefore, the enlarged placental SA in IVP pregnancies suggests an increase in substrate uptake and transport capacity. Newborn IVP calves displayed higher birth weights and plasma fructose concentrations soon after birth, findings which appeared to be associated with clinical and metabolic distress. Our results indicated larger concepti and increased placental fructogenic capacity in mid- to late IVP pregnancies, features which appeared to be associated with an enhanced substrate supply, potentially glucose, to the conceptus.

52 citations

Journal ArticleDOI
TL;DR: The increase in cytoplasmic volume, either by fusion or by aggregation, had a positive effect on embryo development, supporting the establishment of pregnancies and the birth of a viable clone calf after transfer to recipients.
Abstract: Animal cloning has been associated with developmental abnormalities, with the level of heteroplasmy caused by the procedure being one of its potential limiting factors. The aim of this study was to determine the effect of the fusion of hemicytoplasts or aggregation of hemiembryos, varying the final cytoplasmic volume, on development and cell density of embryos produced by hand-made cloning (HMC), parthenogenesis or by in vitro fertilization (IVF). One or two enucleated hemicytoplasts were paired and fused with one skin somatic cell. Activated clone and zona-free parthenote embryos and hemiembryos were in vitro cultured in the well-of-the-well (WOW) system, being allocated to one of six experimental groups, on a per WOW basis: single clone or parthenote hemiembryos (1 × 50%); aggregation of two (2 × 50%), three (3 × 50%), or four (4 × 50%) clone or parthenote hemiembryos; single clone or parthenote embryos (1 × 100%); or aggregation of two clone or parthenote embryos (2 × 100%). Control zona-intac...

47 citations


Cited by
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Journal ArticleDOI
TL;DR: The market for monoclonal antibodies continues to reign supreme, although cellular and gene therapies are slowly starting to gather momentum, andgeoning growth in biosimilars may threaten future brand monopolies.
Abstract: Monoclonal antibodies (mAbs) continue to reign supreme, although cellular and gene therapies are slowly starting to gather momentum. Burgeoning growth in biosimilars may threaten future brand monopolies for mAbs and other biologics.

678 citations

01 Jan 1993
TL;DR: In vitro footprinting indicates that MBD binding can protect a 12 nucleotide region surrounding a methyl-CpG pair, with an approximate dissociation constant of 10(-9) M.
Abstract: MeCP2isachromosomal protein whichbinds toDNA that ismethylated atCpG.Insitu immunofluorescence inmouse cells hasshownthattheprotein ismost concentrated inpericentromeric heterochromatin, suggesting that MeCP2mayplay arole intheformation ofInert chromatin. Herewe haveIsolated a minimal methyl-CpG binding domaln (MBD) fromMeCP2.MBD Is85aminoacids Inlength, andbindsexclusively to DNA thatcontains one or more symmetrically methylated CpGs.MBD hasnegligable non-specific affinity forDNA,confirming thatnon-specific and methyl-CpG specific binding domainsofMeCP2are distinct. Invitrofootprlnting Indicates thatMBDbinding can protect a 12nucleotide regionsurrounding a methyl-CpG pair, withan approximate dissociation constant of10-9M.

582 citations

Journal ArticleDOI
TL;DR: The term 'abnormal offspring syndrome (AOS)' is introduced and a classification system of developmental outcomes is proposed to facilitate research efforts on the mechanisms of the various abnormal phenotypes.

383 citations

10 Feb 2013
TL;DR: The work presented in this article was presented at the Genomic instability and DNA repair workshop of the Keystone Symposia 2013, celebrated in Banff, Alberta (Canada) del 3 al 8 de marzo de 2013.
Abstract: Trabajo presentado en el Genomic Instability and DNA Repair, Keystone Symposia, celebrado en Banff, Alberta (Canada) del 3 al 8 de marzo de 2013

327 citations

Journal ArticleDOI
TL;DR: A good knowledge of the normal physiology of insulin is needed to measure the in vivo insulin resistance of dairy cows.
Abstract: Glucose is the molecule that drives milk production, and insulin plays a pivotal role in the glucose metabolism of dairy cows. The effect of insulin on the glucose metabolism is regulated by the secretion of insulin by the pancreas and the insulin sensitivity of the skeletal muscles, the adipose tissue, and the liver. Insulin resistance may develop as part of physiologic (pregnancy and lactation) and pathologic processes, which may manifest as decreased insulin sensitivity or decreased insulin responsiveness. A good knowledge of the normal physiology of insulin is needed to measure the in vivo insulin resistance of dairy cows.

293 citations