Luciano Zardi

TL;DR: A modified Neuhoff's colloidal Coomassie Blue G‐250 stain is reported, dubbed “blue silver” on account of its considerably higher sensitivity, approaching the one of conventional silver staining, and its full compatibility with mass spectrometry of eluted polypeptide chains is confirmed.

TL;DR: It is reported here that ED-A FN deposition precedes α-SM actin expression by fibroblasts during granulation tissue evolution in vivo and after TGFβ1 stimulation in vitro, and a hitherto unknown mechanism of cytokine-determined gene stimulation based on the generation of an ECM-derived permissive outside in signaling is identified.

TL;DR: A large repertoire of functional antibodies with similar properties was produced by appending short variable complementarity-determining region 3 (CDR3) onto the two antibody germ line segments most frequently found in human antibodies by concentrating sequence diversity in regions of the antibody structure that are centrally located in the antigen binding site.

TL;DR: Results demonstrate that, in addition to their ability to activate other MMP, MT-MMP degrade a number of extracellular matrix macromolecules, and their location at the surface of cells implies that they could play a significant role in the modulation of cell-matrix interactions.

TL;DR: The results show that while in normal, adult, human tissues total FN has a widespread distribution, the B-FN isoform is restricted only to synovial cells, to some vessels and areas of the interstitium of the ovary, and to the myometrium, and the results demonstrate that, in vivo, different FN isoforms have a differential distribution.