Lucimeri Paulino Machado Magalhães

Bio: Lucimeri Paulino Machado Magalhães is an academic researcher from Federal University of Pernambuco. The author has contributed to research in topics: Antibacterial activity & Hyptis. The author has an hindex of 3, co-authored 3 publications receiving 24 citations.

Evaluation of cytotoxic, immunomodulatory and antibacterial activities of aqueous extract from leaves of Conocarpus erectus Linnaeus (Combretaceae).

Abstract: Objectives This work evaluated the antibacterial activity, cytotoxicity and immunomodulatory effect on human peripheral blood mononuclear cells (PBMCs) promoted by aqueous extract from Conocarpus erectus leaves (AELCe). Methods The extract was characterized by thin layer chromatography and ultraperformance liquid chromatography coupled to mass spectrometry (UPLC-MS). Cytotoxicity of AELCe (6.25-50 μg/ml) was investigated using annexin V and propidium iodide. Cytokine and nitric oxide levels in PBMCs culture supernatants exposed or not to AELCe (12.5 μg/ml) were determined, and antibacterial activity was evaluated by disc diffusion and broth microdilution methods. Key findings AELCe contained 3',4'-OH flavonoids, phenylpropanoglycosides, saponins, polymeric proanthocyanidins and hydrolysable tannins. Moreover, 10 other compounds were identified through UPLC-MS technique. AELCe did not affect lymphocyte viability at 6.25 and 12.5 μg/ml. IL-2, IL-10, TNF-α, IFN-γ and nitric oxide was produced in higher levels by cells treated with AELCe. Proliferation and activation of CD8+ T lymphocytes were also stimulated. AELCe showed bacteriostatic activity against clinical and antibiotic-resistant strains of Staphylococcus aureus (MIC between 250 and 1000 μg/ml). Conclusions AELCe showed a moderate bacteriostatic activity and promoted an immunomodulatory status through higher production of Th1 cytokines, nitric oxide release and T CD8+ lymphocytes stimulation.

Antitumor Activity of Leaves from Hyptis mutabilis (A. Rich.) Briq. (Lamiaceae) in Mice Bearing Tumor

Abstract: The Hyptis genus has more than 400 species, many of them being used in folk medicine to treat several conditions. Some anticancer compounds have been isolated from plants of this genus, and for that reason we decided to investigate the potential in vivo antitumor activity of extracts of leaves of Hyptis mutabilis with different polarities (hexane, methanol, water, and hot water) against two mice tumors: sarcoma 180 and Ehrlich solid tumor. Phytochemical analysis revealed strong presence of steroids, saponins, flavonoids (mainly dihydroflavanols), and catechins. Acute toxicity was perfomed according to the up-and-down method showing LD50 values ranging from 100 up to 2500 mg/kg. Antitumor activity was investigated using 10% of the LD50 for each extract. Methotrexate was used as positive control. Both aqueous extracts showed strong inhibition of tumor growth with values up to 70% of inhibition growth for sarcoma 180. Ehrlich solid tumor was only slight inhibited by hexane extract (38.6%). In conclusion, the aqueous extracts of H. mutabilis showed promising results against sarcoma 180 mice tumor.

ESPÉCIES DE Lippia (VERBENACEAE), SEU POTENCIAL BIOATIVO E IMPORTÂNCIA NA MEDICINA VETERINÁRIA E AQUICULTURA.

Abstract: The plant species of the genus Lippia Linn. belonging to the Family of Verbenaceae, and have a great geographic distribution and are easily found in tropical countries. In Brazil, occur mainly Lippia alba Mill.) N. E. Brown, Lippia gracilis Schauer, Lippia grandis Schau, Lippia origanoides Kunth, Lippia sidoides Cham. and Lippia triplinervis Gardner. Since long time, several species are used in popular medicine due to their biological activities and therapeutic. For this reason, different species of Lippia has been studied by its bioactive potential to different diseases in human and animals. The aim of this study was to gather and discuss literature data on the use of Lippia spp. in veterinary medicine and aquaculture. The major constituents of these plants are thymol, carvacrol, geranial, linalool, p-cymene, carvone, neral, limonene, β-caryophyllene, caryophyllene oxide, myrcene and γ-terpinene. However, concentrations of constituents may vary depending on various factors discussed here. In vivo and in vitro assay using mainly L. alba , L. sidoides , L. gracilis , L. origanoides and L. gracilis has reported antimicrobial, antiparasitic, anti-inflammatory, analgesic, anesthetic, and antitumor activities in animals, indicating great potential of these species for the veterinary medicine. Some species of Lippia have been also used in aquaculture, and products obtained from these plants are promising resources, requiring studies for development of technologies that enable their use in the animal health and production. Keywords: Nature product; essential oil; parasites; treatment.

As plantas medicinais da Relação Nacional de Plantas Medicinais de Interesse ao Sistema Único de Saúde (RENISUS) com potencial antifúngico

Abstract: RESUMO| Introdução: Fungos podem causar doenças graves em plantas, animais e seres humanos. Plantas apresentam uma vasta gama de metabólitos secundários, os quais são cada vez mais estudados para suas aplicações terapêuticas, inclusive em relação ao seu potencial antifúngico. Objetivo: Esta revisão sistemática objetivou quantificar os estudos clínicos que referem potencial antifúngico a partir do estudo de plantas constantes na Relação Nacional de Plantas Medicinais de Interesse ao Sistema único de Saúde (RENISUS), publicados entre 2010 e fevereiro de 2013 em três bases de dados científicas (SciELO, Science Direct e Springer). Métodos: Foram lidos inicialmente os títulos dos 21.357 artigos encontrados nas bases de dados, e os artigos eleitos foram posteriormente avaliados quanto aos resumos. Por fim, com os artigos escolhidos nessa etapa, foi analisado o texto completo a fim de selecionar os artigos de interesse com potencial antifúngico. Resultados: Tal análise resultou na seleção de 17 estudos de interesse, com 15 diferentes plantas da RENISUS. Conclusão: Os resultados encontrados neste estudo contribuem para que sejam tratadas novas discussões sobre alternativas terapêuticas aos tratamentos convencionais para fungos patógenos, empregando plantas como um adjuvante.

Toxicity and antitumor potential of Mesosphaerum sidifolium (Lamiaceae) oil and fenchone, its major component

Abstract: The essential oil from Mesosphaerum sidifolium (L’Herit.) Harley & J.F.B.Pastore (syn. Hyptis umbrosa), Lamiaceae (EOM), and its major component, have been tested for toxicity and antitumor activity. EOM was obtained from aerial parts of M. sidifolium subjected to hydro distillation, and gas chromatography-mass spectrometry was used to characterize the EOM chemical composition. The toxicity was evaluated using haemolysis assay, and acute toxicity and micronucleus tests. Ehrlich ascites carcinoma model was used to evaluate the in vivo antitumor activity and toxicity of EOM (50, 100 and 150 mg/kg), and fenchone (30 and 60 mg/kg) after 9 d of treatment. The EOM major components were fenchone (24.8%), cubebol (6.9%), limonene (5.4%), spathulenol (4.5%), β-caryophyllene (4.6%) and α-cadinol (4.7%). The HC50 (concentration producing 50% haemolysis) was 494.9 μg/mL for EOM and higher than 3000 μg/mL for fenchone. The LD50 for EOM was approximately 500 mg/kg in mice. The essential oil induced increase of micronucleated erythrocytes only at 300 mg/kg, suggesting moderate genotoxicity. EOM (100 or 150 mg/kg) and fenchone (60 mg/kg) reduced all analyzed parameters (tumor volume and mass, and total viable cancer cells). Survival also increased for the treated animals with EOM and fenchone. For EOM 150 mg/kg and 5-FU treatment, most cells were arrested in the G0/G1 phase, whereas for fenchone, cells arrested in the S phase, which represents a blockage in cell cycle progression. Regarding the toxicological evaluation, EOM induced weight loss, but did not induce hematological, biochemical or histological (liver and kidneys) toxicity. Fenchone induced decrease of AST and ALT, suggesting liver damage. The data showed EOM caused in vivo cell growth inhibition on Ehrlich ascites carcinoma model by inducing cell cycle arrest, without major changes in the toxicity parameters evaluated. In addition, this activity was associated with the presence of fenchone, its major component.