L
Luhan Yang
Researcher at Harvard University
Publications - 48
Citations - 14393
Luhan Yang is an academic researcher from Harvard University. The author has contributed to research in topics: Genome editing & Cas9. The author has an hindex of 21, co-authored 41 publications receiving 12635 citations. Previous affiliations of Luhan Yang include Wyss Institute for Biologically Inspired Engineering & Massachusetts Institute of Technology.
Papers
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Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
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CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering.
Prashant Mali,John Aach,P. Benjamin Stranges,Kevin M. Esvelt,Mark Moosburner,Sriram Kosuri,Luhan Yang,George M. Church,George M. Church +8 more
TL;DR: This system is engineer to enable RNA-guided genome regulation in human cells by tethering transcriptional activation domains either directly to a nuclease-null Cas9 protein or to an aptamer-modified single guide RNA (sgRNA).
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Modeling the mitochondrial cardiomyopathy of Barth syndrome with induced pluripotent stem cell and heart-on-chip technologies
Gang Wang,Megan L. McCain,Luhan Yang,Aibin He,Francesco S. Pasqualini,Ashutosh Agarwal,Hongyan Yuan,Dawei Jiang,Donghui Zhang,Lior Zangi,Judith Geva,Amy E. Roberts,Qing Ma,Jian-Ping Ding,Jinghai Chen,Da-Zhi Wang,Kai Li,Jiwu Wang,Ronald J.A. Wanders,Wim Kulik,Frédéric M. Vaz,Michael A. Laflamme,Charles E. Murry,Kenneth R. Chien,Richard I. Kelley,George M. Church,Kevin Kit Parker,William T. Pu +27 more
TL;DR: This study combined patient-derived and genetically engineered induced pluripotent stem cells (iPSCs) with tissue engineering to elucidate the pathophysiology underlying the cardiomyopathy of Barth syndrome, a mitochondrial disorder caused by mutation of the gene encoding tafazzin (TAZ).
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Inactivation of porcine endogenous retrovirus in pigs using CRISPR-Cas9.
Dong Niu,Hong-Jiang Wei,Lin Lin,Haydy George,Tao Wang,I-Hsiu Lee,Hong-Ye Zhao,Yong Wang,Yinan Kan,Ellen Shrock,Emal Lesha,Gang Wang,Yonglun Luo,Yubo Qing,Deling Jiao,Heng Zhao,Xiaoyang Zhou,Shouqi Wang,Hong Wei,Marc Güell,George M. Church,George M. Church,Luhan Yang +22 more
TL;DR: The value ofPERV inactivation to prevent cross-species viral transmission is highlighted and the successful production of PERV-inactivated animals to address the safety concern in clinical xenotransplantation are demonstrated.
Journal ArticleDOI
Genome-wide inactivation of porcine endogenous retroviruses (PERVs)
Luhan Yang,Luhan Yang,Marc Güell,Marc Güell,Dong Niu,Haydy George,Emal Lesha,Dennis Grishin,John Aach,Ellen Shrock,Weihong Xu,Jürgen Poci,Rebeca Cortazio,Robert A. Wilkinson,Jay A. Fishman,George M. Church,George M. Church +16 more
TL;DR: This study shows that CRISPR-Cas9 multiplexability can be as high as 62 and demonstrates the possibility that PERVs can be inactivated for clinical application of porcine-to-human xenotransplantation and demonstrated a >1000-fold reduction in PERV transmission to human cells, using engineered cells.