L
Luke W. Koblan
Researcher at Broad Institute
Publications - 26
Citations - 6241
Luke W. Koblan is an academic researcher from Broad Institute. The author has contributed to research in topics: Genome editing & Progeria. The author has an hindex of 13, co-authored 25 publications receiving 2860 citations. Previous affiliations of Luke W. Koblan include Howard Hughes Medical Institute & Harvard University.
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Journal ArticleDOI
Search-and-replace genome editing without double-strand breaks or donor DNA
Andrew V. Anzalone,Andrew V. Anzalone,Andrew V. Anzalone,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Jessie Rose Davis,Jessie Rose Davis,Jessie Rose Davis,Alexander A. Sousa,Alexander A. Sousa,Alexander A. Sousa,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Jonathan M. Levy,Jonathan M. Levy,Jonathan M. Levy,Peter J. Chen,Peter J. Chen,Peter J. Chen,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Aditya Raguram,Aditya Raguram,Aditya Raguram,David R. Liu,David R. Liu,David R. Liu +32 more
TL;DR: A new DNA-editing technique called prime editing offers improved versatility and efficiency with reduced byproducts compared with existing techniques, and shows potential for correcting disease-associated mutations.
Journal ArticleDOI
Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
TL;DR: This work analyzes key considerations when choosing genome editing agents and identifies opportunities for future improvements and applications in basic research and therapeutics.
Journal ArticleDOI
Improving cytidine and adenine base editors by expression optimization and ancestral reconstruction.
Luke W. Koblan,Jordan L. Doman,Jordan L. Doman,Jordan L. Doman,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Jonathan M. Levy,Jonathan M. Levy,Jonathan M. Levy,Tristan Tay,Tristan Tay,Tristan Tay,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Juan Pablo Maianti,Juan Pablo Maianti,Juan Pablo Maianti,Aditya Raguram,Aditya Raguram,Aditya Raguram,David R. Liu,David R. Liu,David R. Liu +24 more
TL;DR: It is shown that expression levels are a bottleneck in base-editing efficiency, and cytidine and adenine base editors are optimized by modification of nuclear localization signals and codon usage, and ancestral reconstruction of the deaminase component.
Journal ArticleDOI
Improved base excision repair inhibition and bacteriophage Mu Gam protein yields C:G-to-T:A base editors with higher efficiency and product purity.
Alexis C. Komor,Alexis C. Komor,Alexis C. Komor,Kevin T. Zhao,Kevin T. Zhao,Kevin T. Zhao,Michael S. Packer,Michael S. Packer,Michael S. Packer,Nicole M. Gaudelli,Nicole M. Gaudelli,Nicole M. Gaudelli,Amanda L. Waterbury,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Y. Bill Kim,Y. Bill Kim,Y. Bill Kim,Ahmed H. Badran,Ahmed H. Badran,Ahmed H. Badran,David R. Liu,David R. Liu,David R. Liu +24 more
TL;DR: Fourth-generation base editors (BE4 and SaBE4) are engineered that increase the efficiency of C:G to T:A base editing by approximately 50%, while halving the frequency of undesired by-products compared to BE3, and recommend their use in future efforts.
Journal ArticleDOI
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Michelle Richter,Kevin T. Zhao,Kevin T. Zhao,Kevin T. Zhao,Elliot Eton,Elliot Eton,Elliot Eton,Audrone Lapinaite,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Benjamin W. Thuronyi,Christine D. Wilson,Christine D. Wilson,Christine D. Wilson,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,Jing Zeng,Daniel E. Bauer,Daniel E. Bauer,Jennifer A. Doudna,David R. Liu,David R. Liu,David R. Liu +24 more
TL;DR: AB8e augments the effectiveness and applicability of adenine base editing and can efficiently install natural mutations that upregulate fetal hemoglobin expression in the BCL11A enhancer or in the the HBG promoter in human cells, targets that were poorly edited with ABE7.10.