Author
M. Burstein
Bio: M. Burstein is an academic researcher from Albert Einstein College of Medicine. The author has contributed to research in topics: Very low-density lipoprotein & Chylomicron. The author has an hindex of 6, co-authored 7 publications receiving 3507 citations.
Papers
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TL;DR: With a single preparative ultracentrifugation, immunologically pure high density lipoproteins can be isolated from large volumes of serum.
2,449 citations
TL;DR: A simple precipitation method for the separation of HDL subclasses in human plasma found that HDL2 is the more variable component and may be a more meaningful index of altered HDL metabolism.
805 citations
TL;DR: In this article, the authors discuss lipoprotein-polyanion-metal interactions and show that the formation of an insoluble complex is more easily achieved with chylomicrons and VLDL than with LDL, and in turn more easily with LDL than with HDL.
Abstract: Publisher Summary This chapter discusses lipoprotein–polyanion–metal interactions. Virtually all the plasma lipids are combined with specific proteins in the form of lipoproteins. In the presence of divalent cations, sulfated polysaccharides and sodium phosphotungstate can precipitate at neutral pH all the lipoprotein classes, namely, chylomicrons, very low-density lipoproteins (VLDL), low density lipoproteins (LDL), and high-density lipoproteins (HDL). This precipitation is dependent on the nature of the anion and cation, the concentration of reagents, and the ionic strength. The formation of an insoluble complex is more easily achieved with chylomicrons and VLDL than with LDL, and in turn more easily with LDL than with HDL. This is related to the protein:lipid ratio and not to the composition of the lipid moiety of the individual lipoproteins. Under certain conditions, the precipitation of LDL is complete and selective. By varying the ionic strength, differential precipitation of chylomicrons, VLDL, and LDL, followed by turbidimetric estimation of these lipoproteins, can be accomplished. Serum lipoproteins are also precipitated by divalent cations and polyphosphates, tetracyclines, or anionic detergents.
255 citations
TL;DR: This precipitation requires the presence of a non-dialyzable thermolabile serum factor and the precipitated lipoproteins float on centrifugation and a clear subnatant is obtained.
54 citations
Journal Article•
18 citations
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TL;DR: A simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low- density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+.
Abstract: We describe a simplified method for measuring high-density lipoprotein cholesterol in serum after very-low- and low-density lipoproteins have been precipitated from the specimen with sodium phosphotungstate and Mg2+. Values so obtained correlate well with values obtained with the heparin-Mn2+ precipitation technique (r = 0.95, CV less than 5% in 66% of the subjects studied and between 5 and 10% in the remaining ones) or by ultracentrifugal separation (r = 0.82, CV less than 5% in 80% of the subjects studied and between 5 and 10% in the remaining ones). Our precipitation technique is more appropriate for routine clinical laboratory use.
1,802 citations
TL;DR: Determination of the extent of the unprecipitated apoB-associated lipoproteins by sensitive radioimmunoassay and of the amount of precipitated high density lipoprotein by radial immunodiffusion assay of apolipoproteins A-I and A-II at various heparin and Mn(2+) concentrations indicated that the usual Heparin level was adequate.
1,297 citations
TL;DR: Both HDL cholesterol and HDL2 levels were associated with a substantially decreased risk of myocardial infarction, but the HDL3 level was the strongest predictor; the relative risk was 0.3 (95 percent confidence interval, 0.2 to 0.6).
Abstract: Background. The independent contributions of subfractions of high-density lipoprotein (HDL) cholesterol (HDL2 and HDL3) and apolipoproteins in predicting the risk of myocardial infarction are unclear. Prospective data are sparse, but HDL2 is widely believed to be a more important predictor than HDL3. Methods. Blood samples were collected at base line from 14,916 men (ages, 40 to 84 years) who were participants in the Physicians' Health Study. After five years of follow-up, plasma samples from 246 men with new myocardial infarction (case subjects) were analyzed together with specimens from 246 men matched to them for age and smoking status who had not had a myocardial infarction. Results. The levels of total cholesterol and apolipoprotein B-100 were significantly associated with an increased risk of myocardial infarction (data on levels of low-density lipoprotein cholesterol were unavailable). Both HDL cholesterol and HDL2 levels were associated with a substantially decreased risk of myocardial in...
1,199 citations
TL;DR: The relationship of future clinical coronary heart-disease to the plasma-high-density-lipoprotein (H.H.D.L.)-cholesterol concentration has been examined in a 2-year case-control follow-up study of 6595 men aged 20-49 years living in the municipality of Tromsø, Norway and support the proposal that a low H.d.L. cholesterol concentration is important in accelerating the progression of coronary atherosclerosis.
1,180 citations
TL;DR: The inverse association of moderate alcohol intake with the risk of myocardial infarction is confirmed and support the view that the effect is mediated, in large part, by increases in both HDL2 and HDL3.
Abstract: Background Previous studies have suggested that moderate alcohol intake exerts a protective effect against coronary heart disease. Alterations in plasma lipoprotein levels represent one plausible mechanism of this apparent protective effect. Methods We therefore examined the interrelation among alcohol consumption, plasma lipoprotein levels, and the risk of myocardial infarction in 340 patients who had had myocardial infarctions and an equal number of age- and sex-matched controls. The case patients were men or women less than 76 years of age with no history of coronary disease who were discharged from one of six hospitals in the Boston area with a diagnosis of a confirmed myocardial infarction. Alcohol consumption was estimated by means of a food-frequency questionnaire. Results We observed a significant inverse association between alcohol consumption and the risk of myocardial infarction (P for trend, <0.001 after control for known coronary risk factors). In multivariate analyses, the relative risk for ...
1,022 citations