Author
M. Dlaska
Bio: M. Dlaska is an academic researcher. The author has contributed to research in topics: Inductively coupled plasma mass spectrometry & Microwave digestion. The author has an hindex of 1, co-authored 1 publications receiving 68 citations.
Papers
More filters
TL;DR: In this article, a vesicle mediated high performance liquid chromatographic (HPLC)-microwave digestion (MW)-hydride generation (HG) system coupled on-line with AAS and inductively coupled plasma mass spectrometry (ICP-MS) was assessed for selenium species separation and detection.
Abstract: A vesicle mediated high performance liquid chromatographic (HPLC)–microwave digestion (MW)–hydride generation (HG) system coupled on-line with atomic absorption spectrometry (AAS) and inductively coupled plasma mass spectrometry (ICP-MS) has been assessed for selenium species separation and detection. Selenocystine, selenomethionine, selenoethionine, selenourea, SeIV and SeVI are separated by vesicle mediated chromatography prior to on-line selenocompounds microwave digestion with a KBrO3–HBr mixture to generate SeIV continuously, which is finally transformed into SeH2, in a continuous manner with a merging flow of sodium tetrahydroborate(III). Analytical characteristics of this coupling are compared with those obtained coupling HPLC–ICP-MS via conventional nebulisation. Detection limits (DLs) obtained for selenocystine, selenomethionine, selenoethionine, selenite and selenate in spiked human urine, when ICP-MS was used as detector, ranged between 1.0 and 5.3 µg l–1 (51–267 pg), while precision ranged between±3.4 and±8.4%. This continuous system, vesicle mediated HPLC–MW–HG–atomic detection, allows the separation and detection of selenocystine, selenourea, selenomethionine, selenoethionine, selenite and selenate in human urine. The analytical versatility of such coupling (with ICP-MS as the atomic detector) allows basal selenium speciation in urine. Three different normally occurring selenium species in human urine, simply diluted (1+1), have been found. The relative sophistication of the vesicle mediated–HPLC–MW–HG–ICP-MS interface, versus HPLC–ICP-MS via conventional nebulisation, can be justified because of its considerably higher sensitivity for most of the selenocompounds assayed, lower matrix interferences and the possibility of simultaneous interference free 77Se and 78Se monitoring.
68 citations
Cited by
More filters
TL;DR: This review focuses on the basic principles of LC-ICP-MS, its historical development and the many ways in which this technique can be applied.
209 citations
TL;DR: The use of surfactant-based organized assemblies in analytical atomic spectroscopy is extensively and critically reviewed along three main lines: first, the ability of organised media to enhance detection of atomic Spectroscopic methods by favourable manipulation of physical and chemical properties of the sample solution; second, the extension of separation mechanisms by resorting to organized media and third a discussion of synergistic combinations of liquid chromatography separations and atomic detectors via the use of vesicular mobile phases as discussed by the authors.
146 citations
TL;DR: Reversed-phase HPLC-ICP-MS with 0.1% HFBA in the mobile phase allowed more than 20 selenium compounds to be separated in 70 min in an isocratic elution mode; the separation of natural Selenium-enriched sample extracts was examined and explained.
138 citations
TL;DR: This review deals with liquid phase separation of major arsenic and selenium species followed by element specific detection by describing only currently used methods that were or could be applied to As and Se speciation in environmental matrices.
102 citations
TL;DR: Knowledge of the biochemistry of the element coupled with appropriate techniques for the study of the distribution of selenium species in health and disease could help to identify sensitive markers of Selenium status.
99 citations